不同粒度麻杏石甘汤配方药材超微粉中麻黄碱及伪麻黄碱的溶出率比较

Solubilities of ephedrine and d-pseudo-ephedrine from different sizes of ultramicro-particles of the compound traditional Chinese herbs Maxing Shigan decoction

目的:运用反相高效液相法(RP-HPLC)比较不同超微粉碎条件下中药复方麻杏石甘汤样品中的麻黄碱(ephedrine)与伪麻黄碱(d-pseudo-ephedrine)的溶出率变化趋势。方法:YMC ODS-C18柱(4.6 mm×150 mm,4μm);流动相水-乙腈-十二烷基硫酸钠(SDS)-磷酸(650∶350∶5∶1);柱温50℃;检测波长210 nm;流速1.5 mL.min-1。结果:超微粉碎前后样品溶出率差异显著。结论:证明对于麻杏石甘汤复方,经过超微粉碎技术处理后的样品,其麻黄碱与伪麻黄碱的溶出速度比未经超微粉碎处理之复方加快,且溶出率增加。

Objective： To compare the solubilities of ephedrine and d-pseudo-ephdrine in the compound of traditional Chinese drugs--Maxing Shigan decoction with and without ultramicro-pulverizations using a RP-HPLC procedure. Method： The analytical column was a YMC ODS-C18 （4.6 mm × 150mm, 4 μm） . A mixture of water-acetonitrile-sodium dodecyl sulfate-phosphorous acid （650： 350：5：1 ） was used as the mobile phase at flow rate of 1.5 mL·min^-1 The column temperature was 50℃ and the wavelength was 210 nm. Results： In this way, ephedrine and d-pseudo-ephedrine of the compound samples can be separated well and showed significant differences before and after the ultramicro-pulverizations. Conclusion： For the Maxing Shigan decoction, the dissolution rates and solubilites of ephedrine and d-pseudoephedrine ultramicro-pulverization were greatly improved.