黄芩苷、栀子苷及其配伍治疗局灶脑缺血大鼠药效评价及作用机制研究

Pharmacological evaluation of baicalin and jasminoidin and their combination on focal cerebral ischemia-reperfusion injury

目的:探讨清开灵有效组分黄芩苷、栀子苷及其配伍治疗脑缺血大鼠药效及其药理作用。方法:线栓法建立大鼠脑缺血再灌注模型,应用TTC染色、神经功能检查法、病理超微组织观察综合评价黄芩苷、栀子苷及其配伍治疗脑缺血大鼠的功效作用,WESTERN-BLOT法测定Akt-PKB,CREB,PCREB的表达情况。结果与结论:黄芩苷与栀子苷配伍具有神经脑保护作用,其功效优于单一组分治疗组和尼莫地平治疗组,并且对脑海马组织Akt/PKB,CREB,P-CREB蛋白表达有影响,其脑保护的药效加和作用与信号转导PI3K-Akt-PKB-BAD-CREB-PCREB途径有关。

Objective： To elucidate the therapeutic effect and the influence on PI3K-Akt-PKB-BAD-CREB-PCREB pathway in focal cerebral ischemia rat responses before and after treatment with baicalin and jasminoidin given alone or in combination. Method： Rat model of ischemia reperfusion was established with thread. Generally accepted methods were used, including TTC staining, behavior test, as well as micro and ultra microscopy which can dynamically and accurately monitor pathological and physiological changes after cerebral ischemia on earlier period, to evaluate the brain injury induced by ischemia and the attenuations by the drugs. The difference of PI3K-Akt-PKB-BAD-CREB- PCREB expression was detected by western-blot technology. Result and Conclusion：The combination of baicalin and jasminoidin composition can be potential neuroprotective agent. TIC staining technology combined with behavior grade and ultrmicro-stmcture observation on brain tissue is effective method to evaluate protective agent, which is related to signal transduction PI3K-Akt-PKB-BAI）-CREB-PCREB pathway. The resuhs provide benotical basis for revealing the complex of therapeutic mechanism of traditional Chinese medicine Qingkai Ling（QKL）.