丹参注射液在妊娠肝内胆汁瘀积症中的治疗机制研究

Study on therapeutic mechanisms of Danshen injection in intrahepatic cholestasis of pregnancy

目的:探讨丹参注射液在治疗妊娠肝内胆汁瘀积症中的机制。方法:采集妊娠肝内胆汁瘀积症患者血清,与脐静脉内皮细胞共培养模拟其宫内环境,不同含量(1,2,4,8 g.L-1)的丹参注射液进行干预,MTT法检测内皮细胞的活力,ELISA法和免疫细胞化学法分别检测内皮细胞分泌血管内皮生长因子的变化。结果:与空白组相比,丹参注射液治疗组能使脐静脉内皮细胞活力增强,脐静脉内皮细胞自分泌可溶性和膜结合性血管内皮生长因子(VEGF)蛋白增加,并呈现剂量依赖性;以8 g.L-1的作用最佳。结论:丹参注射液对妊娠肝内胆汁瘀积症患者血清刺激后的脐静脉内皮细胞具有保护作用,其保护作用可能与增强血管内皮生长因子的分泌有关。

Objective： To study the therapeutic mechanisms of Danshen injection in intrahepatic cholestasis of pregnancy （ICP）. Method： The experimental serum was collected from 6 patients with ICP before therapy when in hospital. Endothelial cells were isolated from human umbilical veins chosen among the 10 normal pregnancy according to Jaffe＇s method. Serum of 20% concentration of ICP and different concentration of Danshen injection were cocuhured with human vascular endothelial cells （HUVECs） for 24 hours. MTT method was applied to measuring the vitality of HUVECs. The expression value of vascular endothelial growth factor （VEGF） of different groups was detected by immunocytochemistry and enzyme-linked immunosorbent assay （ELISA） respectively. Result： Compared with ICP group, the value of MTT and VEGF in the Danshen injection therapeutic groups were increased with the increase of concentration and moreover, 8 g·L^-1 of Danshen injection was the optical concentration. By immunocytochemistry, the expression of VEGF in HUVECs in the Danshen injection therapeutic groups was also enhanced with the increase of concentration. Conclusion： Danshen injection can protect HUVECs against the injury oflCP serura and promote the expression of VEGF. Danshen injection improves HUVECs by increasing the value of VFGF.