金雀异黄素抑制IL-1α刺激破骨样细胞的组织蛋白酶K表达

Genistein Inhibit Cathepsin K Expression Stimulated by IL-1α in Osteoclast-like Cells

从人骨巨细胞瘤组织中纯化破骨样细胞,用不同浓度的金雀异黄素温育48h,观察IL-1α刺激后1h后组织蛋白酶K表达。结果发现:与阴性对照组相比,IL-1α明显刺激破骨样细胞表达组织蛋白酶K(P<0.01);而金雀异黄素抑制IL-1α刺激后组织蛋白酶K转录及表达,且呈剂量依赖关系(P<0.01);加用雌激素受体拮抗剂ICI182.780后,金雀异黄素作用被部分拮抗。金雀异黄素通过雌激素受体部分抑制IL-1α刺激破骨样细胞的组织蛋白酶K表达。

The osteoclast-like cells （OCLs） isolated from tissue of human giant cell tumor of bone, were stimulated by IL-1α in the presence of genistein. Then the expression of cathepsin K （CK） was determined by RTPCR and Western blot in OCLs. Compared with control group, the expressions of CK were up-regulated after stimulated by IL-1α in other groups （P〈0.01）. Genistein down-regulated CK gene expression stimulated by IL-1α at the transcription level stimulated in dose-dependent manner （P〈0.01） and its effect was abrogated partly after treated with the estrogen receptor antagonist ICI 182.780. Genistein inhibit CK expression stimulated by IL-1α through estrogen receptor in OCLs. We found IL-1α stimulated the expression of CK in OCLs.