乳宁Ⅱ号对小鼠乳腺癌抑瘤、抗转移作用的实验研究

Antineoplastic and Anti-metastasizing Effects of Runing Ⅱ on Breas Cancer in Mice:An Experimental study

目的:研究乳宁Ⅱ号对津白Ⅱ号(TA2)MA891小鼠乳腺癌的抑瘤和抗转移作用。方法:将体外培养的MA891细胞接种在津白Ⅱ号小鼠右腋皮下,接种后将小鼠分为生理盐水(NS)组、环磷酰胺(CTX)组、乳宁Ⅱ号组和CTX+乳宁Ⅱ号组。观察肿瘤抑制率和肺转移率、各组肿瘤细胞周期和凋亡情况。结果:CTX组、乳宁Ⅱ号组、CTX+乳宁Ⅱ号组与NS组比较均能显著抑制肿瘤和肺转移,抑瘤率分别为72.14%、35.1%、73.90%;肺转移抑制率分别为38.03%、34.04%、56.91%。结论:乳宁Ⅱ号能明显抑制肿瘤生长和抗转移,且对CTX有减毒增效的作用。

Objective：To investigate antineoplastie and antimctastasizing effects of Runing Ⅱ prescription on Jinbai Ⅱ （TA2）mouse breast cancer line MA891. Methods：In vitro culture MA891 cells were inoculated subcutanously into Jinbai Ⅱ mice （TA2）. And then the mice were divided into four groups and treated respectively with normal saline,cyclophosphamide （CTX） ,Runlng Ⅱ and cyclophosphamide（CTX） plus Runing Ⅱ. The growth of tumors and lung metastasis were observed and compared among the four groups. Results：Tumor inhibition rates were 72.14% .35.1%,73.90% in CTX group.Runing Ⅱ group and CTX ＋ Runing Ⅱ group, respectively;the inhibition rates of lung metastases were 38.03% ,34.04% ,56.91% , respectively. Conclusion： Runing Ⅱ prescription could inhibit the growth of the tumor and reduce the lung metastases. And it could also reduce the toxicity of CTX abd enhance its therapeutic effectiveness.