尾核内注射L-精氨酸对大鼠血浆和脑组织中cGMP含量的影响

Effect of Administration of L-arginine into Caudate Nucleus on Contents of Cgmpin Rats Plasm and Brain

目的观察一氧化氮(NO)对大鼠血浆和脑组织中cGMP的含量的影响。方法尾核内微量注射一氧化氮(NO)的前体L-精氨酸(L-Arg)和鸟苷酸环化酶(GC)抑制剂亚甲基蓝(MB),以放射免疫方法测定注药后5、10、15、20、30min血浆和脑组织中cGMP含量的变化。结果大鼠尾核内微量注射L-Arg,血浆和脑组织中cGMP的含量明显升高,微量注射MB后大鼠血浆和脑组织中cGMP含量显著降低,20min内与对照组相比差异均有统计学意义(P<0.01或P<0.05)。结论提示中枢神经系统内NO可提高血浆和脑组织中cGMP的含量。

Objective To observe the effects of nitric oxide（NO） on contents of guanosine 3＇ ：5＇ - cyclic monophosphate（cGMP） in plasma and brain of rats. Methods Radioimmunoassay was used to measure cGMP in plasma and brain by micro- injecting L- arginine（L- Arg） ,and methylene blue（MB） into rat caudate nucleus at 5,10,15,20 and 30 minuts. Results cGMP level Of plasma and brain was increased significantly When micro- injecting L - Arg but it was decreased significantly when micro - injecting MB. There were significant differences With Contrast groups in 20 minuts（P 〈 0.01 Or P 〈 0.05）. Conclusion NO in central nervous system（CNS） can increase cGMP level in plasm and brain of rats.