脑胶质瘤低氧诱导因子鄄1α的表达及其意义

Expression of hypoxia-inducible factor-1α and its role in glioma

目的探讨低氧诱导因子鄄1α在脑胶质瘤中的表达及其意义。方法60例脑胶质瘤标本中Ⅰ级11例(毛细胞型星形细胞瘤8例、脉络丛乳头状瘤2例、黏液乳头状型室管膜瘤1例);Ⅱ级20例(弥漫型星形细胞瘤11例、少突胶质细胞瘤5例、室管膜瘤2例、多形性黄色瘤型星形细胞瘤2例);Ⅲ级21例(间变性星形细胞瘤12例、间变性少突胶质细胞瘤6例、间变性室管膜瘤3例);Ⅳ级8例(均为胶质母细胞瘤)。采用免疫组织化学方法检测胶质瘤标本中低氧诱导因子鄄1α的表达变化,并与胶质瘤体积及患者年龄、性别进行统计学分析。结果(1)胶质瘤组织低氧诱导因子鄄1α表达呈阳性反应,主要位于细胞质和(或)细胞核,具有明显的异质性;肿瘤浸润边缘部的肿瘤细胞表达明显增强;而阴性对照标本和10例对照脑组织标本则无表达。胶质瘤组织中低氧诱导因子鄄1α阳性表达率为71.67%(43/60),其中Ⅰ级为27.27%(3/11),Ⅱ级70.00%(14/20),Ⅲ级85.71%(18/21),Ⅳ级100%(8/8)。高级别胶质瘤者低氧诱导因子鄄1α阳性表达率明显高于低级别者,不同级别间差异有高度统计学意义(χ2=15.907,P<0.01);表达强度与病理级别间呈高度正相关(rs=0.480,P<0.01)。(2)低氧诱导因子鄄1α表达与患者年龄、性别及原发肿瘤体积的大小等均无相关性(均P>0.05)。结论脑胶质瘤低氧诱导因子鄄1α的表达强弱与肿瘤病理分级相关。

Objective To investigate the expression of hypoxia inducible factor-1α （HIF-let） and its significance in glioma. Methods A total of 60 glioma specimens including 11 cases of grade Ⅰ （8 pilocytic astrocytoma, 2 choroids plexus papillomas, 1 myxo-papillary ependymoma）, 20 cases of grade Ⅱ （11 diffuse astrocytoma, 5 oligodendroglioma, 2 ependymoma, 2 pleomorphic xanthoastrocytoma）, 21 cases of grade Ⅲ （12 anaplastic astrocytoma, 6 anaplastic oligodendroglioma, 3 anaplastic ependymoma） and 8 cases of grade Ⅳ （glioblastoma）, were enrolled in the study. Immunohistochemical assay was carried out to detect the expression levels of HIF-1α, and the results were analyzed statistically in correlation with the volumes of glioma as well as the age and sex of patients. Results 1） There was positive HIF-1α expression in the glioma specimens, mostly observed in the cytoplasm and/or nucleus with obvious heterogeneity. Siginifcantly enhanced expression could be found in the margin of infiltration areas of the tumor. The negative expression was found in the negatively-controlled specimens and other 10 specimens of normal brain tissue. The positive HIF-1α expression rate for the glioma specimens was 71.67% （43/60）, among them, 27.27% （3/11） for grade Ⅰ , 70.00% （14/20） for grade Ⅱ, 85.71 （18/21） for grade Ⅲ and 100% （8/8） for grade Ⅳ, respectively. The positive expression rate of HIF in higher pathological grade glioma was obviously higher than that in lower pathological grade glioma. And the differences were statistically significant （X^2=15.907, P〈 0.01）. The expression intensity was positively correlated with the pathological grade （rn=0.480, P〈 0.01） of glioma. 2） The HIF-1αexpression was not correlated with the age and sex of the patients and the volum of tumors （P〉0.05）. Condusion The HIF-1α expression is correlated with the pathological grade of glioma.