蚕丝丝素纤维的制备及其与骨髓间充质干细胞相容性的研究

Research on Preparation of Silk Fibroin and Its Biocompatibility with Rat Bone Marrow Mesenchymal Stem Cells

组织工程为韧带损伤修复提供了可行途径,但韧带修复对支架材料各方面性能要求都很高。在力学性能方面,不仅要求材料有一定的强度而且需要有良好的韧性。在满足力学性能的同时,支架材料还必须兼具优良的生物相容性。蚕丝作为一种天然生物蛋白质,由于其良好的力学性能显示了在组织工程方面应用的前景。但由于丝胶存在污染问题,因此脱胶成为蚕丝在医学领域应用的首要问题。本实验首先比较了三种脱胶试剂对蚕丝力学性质的影响,选择了影响最小的碳酸钠作为脱胶试剂,进而确定了碳酸钠脱胶的最佳条件为:试剂浓度0.4%,温度90℃,时间为1 h。然后在脱胶后的丝素纤维上种植了大鼠骨髓间充质干细胞(R at bone m arrow m esenchym a l stemce lls,rM SC s),通过扫描电镜(SEM)、荧光显微镜检测了丝素上细胞的生长情况,结果显示蚕丝具有良好的生物相容性,细胞亲和力。为蚕丝在韧带组织工程方面的进一步应用奠定了基础。

The newly developed approach of tissue engineering has been shown to be great potential on the ligament reconstruction, however, the criterion for the scaffolding material was strict. The scaffold material must have enough strength as well as elasticity, at the same time, it should be biocompatible. As a nature protein, silk is a promising tissue engineering scaffold material for its excellent mechanical property. However, because of the contamination of sericin, the chief problem of silk＇s medical use is degumming. We compared three degumming reagents to choose the one which has least effect on the mechanical property of silk, and then the best degumming condition was confirmed： 0. 4~NazCO3, 90＂C, 1 h. Rat bone morrw mesenchymal stem cells （rMSCs） were seeded on the fibroin, and scanning electron microscope （SEM） and fluorescence microscope were used to detect the biocompatibility of it. And the results showed that fibroin had outstanding biocompatibility and cell affinity, which indicated the further use of fibroin in tissue engineering.