人鼻咽癌克隆株体外传代过程的群体变化

THE POPULATION VARIANCE OF HUMAN NASOPHAR- YNGEAL CARCINOMA CLONAL STRAIN (CNE-2Z-5-2-B 7) DURING PASSAGES IN VITRO

将人低分化鼻咽癌克隆株CNE-2 Z-5-2-B_7在体外连续传代培养,从群体的角度观察了不同代数的细胞形态定量、DNA含量和体外增殖能力的变化。结果:(1)各细胞形态参数、DNA含量出现异质性,为多种瘤细胞亚群所构成,而且随传代过程变化、消长。(2)41代以细胞面积大、核大、核浆比小、DNA含量高、异质性明显的大细胞群体占优势。其形态特征与鼻咽低分化鳞癌的大核型相似。(3)81代主要为胞、核面积小、核浆比大、DNA含量高的小细胞群体,其体外增殖能力较第1代明显为高,形态及生长特征与鼻咽未分化癌相似。提示随着肿瘤的演进,如不给予影响(治疗),瘤细胞有恶性发展的内在倾向。

The clonal strain (CNE-2 Z-5-2-B 7) which derived from human poorly differentiated na-sopharyngeal carcinoma (NPC) cell line CNE-2 Z was serial cultured from primary to the 81th passage in vitro. The cell morphometric, nuclear DNA content and the proliferation in vitro of different passages were investigated. The results indicated (1) During culture passaged in vitro, the cell morphometric and nuclear DNA content showed their heterogenecity and construction by different subpopulations that would appeared or disappeared. (2) The subpopu-lation with large cellular and nuclear area but low N/C ratio, high DNA content reached the top on the 41 th passage, which were similar to characterestics of the large nucleus type of poorly differetiated squarmous carcinoma. (3) The cell population became mainly small cellular and nuclear area, high N/C and DNA content with higher proliferation on the 81th passage than that on primary clonal strain, which were as undifferentiated carcinoma.