摘要
水牛卵母细胞体外成熟22 h后,分别用盲吸法、点击法和纺锤体成像系统(Spindleview System)去核。结果,三者的去核率分别为65.1%、81.8%和95.0%,差异极显著(P< 0.01)。以水牛胎儿成纤维细胞为供体,通过核移植构建的重构胚的融合率、分裂率和囊胚发育率, 在上述3种方法之间均没有显著差异(P>0.05)。认为点击法是一种较为简单、实用和有效的去核方法。
Buffalo oocytes matured in vitro for 22 h were enucleated by blind-sucking, point-hitting and Spindle-View System, respectively. The enucleation rate of oocytes by the Spindle-View System (95%) was significantly(P〈0. 01) higher than that achieved by blind-sucking(65. 1%) and the pointhitting(81.8%). However, there was no significant difference in fusion ration, cleavage rate and blastocyst yield of the reconstructed embryos among the three enucleation methods(P〉0.05). Results indicated that the point-hitting can he employed for enucleation of oocytes in consideration of the costs of the Spindle-View System.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第6期493-496,共4页
Chinese Veterinary Science
基金
国家自然科学基金资助项目(30460090)
关键词
水牛
卵母细胞
去核
核移植
点击法
buffalo
oocyte
enucleation
nuclear transplantation
point-hitting