水牛卵母细胞去核方法的比较

Comparison of enucleation protocols of buffalo oocytes

水牛卵母细胞体外成熟22 h后,分别用盲吸法、点击法和纺锤体成像系统(Spindleview System)去核。结果,三者的去核率分别为65．1％、81．8％和95．0％,差异极显著(P< 0．01)。以水牛胎儿成纤维细胞为供体,通过核移植构建的重构胚的融合率、分裂率和囊胚发育率, 在上述3种方法之间均没有显著差异(P>0．05)。认为点击法是一种较为简单、实用和有效的去核方法。

Buffalo oocytes matured in vitro for 22 h were enucleated by blind-sucking, point-hitting and Spindle-View System, respectively. The enucleation rate of oocytes by the Spindle-View System （95%） was significantly（P〈0. 01） higher than that achieved by blind-sucking（65. 1%） and the pointhitting（81.8%）. However, there was no significant difference in fusion ration, cleavage rate and blastocyst yield of the reconstructed embryos among the three enucleation methods（P〉0.05）. Results indicated that the point-hitting can he employed for enucleation of oocytes in consideration of the costs of the Spindle-View System.