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可溶性支架转染c-myc基因反义寡核苷酸对静脉移植物c-myc及PCNA表达的影响

Effect on the Expression of C-myc and Proliferating Cell Nuclear Antigen of Vein Grafts Transferred by C-myc Antisense Oligodeoxynucleotides of Soluble Stent
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摘要 目的采用可溶性支架将原癌基因c-m yc反义寡核苷酸转染静脉移植物,观察其对静脉移植物内c-m yc蛋白和增殖细胞核抗原(PCNA)蛋白产物表达的影响。方法50只新西兰大耳白兔建立兔颈外静脉颈总动脉旁路移植模型后随机分成5组,每组10只。对照组:无支架;组1:植入单纯可溶性支架;组2:植入正义c-m yc寡核苷酸可溶性支架;组3:植入反义c-m yc寡核苷酸可溶性支架;组4:植入不匹配c-m yc寡核苷酸可溶性支架。于静脉移植后7d、28d和90d取出静脉移植物,采用免疫组织化学方法检测其c-m yc蛋白和PCNA蛋白的表达。结果对照组、组1、组2、组4静脉移植术后7d、28d、90d时静脉移植物内膜和中膜内可见大量c-m yc蛋白和PCNA蛋白表达阳性的细胞,与同时间点组3比较差别均有统计学意义(P<0.01)。28d、90d时5组静脉移植物内c-m yc蛋白的表达与同组7d时比较明显增强(P<0.01)。结论可溶性支架转染c-m yc反义寡核苷酸可显著抑制静脉移植物内c-m yc蛋白和PCNA蛋白的表达。 Objective To investigate the effect on expression of c-myc and proliferating cell nuclear antigen (PCNA) of vein grafts transferred by c-myc antisense oligodeoxynucleotides(ODN) of soluble stent. Methods A rabbit model of common carotid arteries grafted by external jugular veins was constructed in 50 New Zealand rabbits and were randomly divided into five groups, 10 rabbits each group. Control group: no stents ; group 1: soluble stent ; group 2: soluble stent with sense-ODN; group 3: soluble stent with antisense-ODN; group 4.. soluble stent with mismatch-ODN. At 7 d, 28 d and 90 d after surgery, vein grafts were harvested. The expression of c-myc and PCNA were identified by immunochemistry methods. Results At 7d, 28d, 90d after surgery, the expression of c-myc and PCNA of the intima and media of vein grafts in control group, group 1, group 2, group 4 were higher significantly than that in group 3 (P〈0. 01). At 28d, 90d after surgery, the expression of c-myc in five groups were higher than that in the same group at 7d after surgery (P〈0. 01). Conclusion Soluble stent can transfer ODN effectively. C- myc antisense-ODN transferred by soluble stent can inhibit significantly the expression of c-myc and PCNA in the intima and media of vein grafts.
出处 《中国胸心血管外科临床杂志》 CAS 2006年第3期176-179,共4页 Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金 国家自然科学基金资助项目(30371408)~~
关键词 c—myc 增殖细胞核抗原 可溶性支架 反义寡核苷酸 c-myc Proliferating cell nuclear antigen Soluble stent Antisense oligodeoxynucleotides
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参考文献11

  • 1黄志雄,郭加强,李晓惠,赵秀文.可溶性支架转移基因的实验研究[J].中华外科杂志,1997,35(5):310-312. 被引量:13
  • 2Owens GK, Kumar MS, Wamhoff BR. Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev, 2004,84(3) : 767-801.
  • 3Regan CP, Adam PJ, Madsen CS, et al. Molecular mechanisms of decreased smooth muscle differentiation marker expression after vascular injury. J Clin Invest, 2000,106(9):1139-1147.
  • 4Edelman ER, Simons M, Sirois MG, et al. C-myc in vasculoproliferative disease. Circ Res, 1995, 76(2) :176-182.
  • 5Mannion JD, Ormont ML, Magno MG, et al. Sustained reduction of neointima with c-myc antisense oligonucleotides in saphenous vein grafts. Ann Thorac Surg, 1998, 66 (6): 1948-1952.
  • 6Forte A, Galderisi U, De Feo M, et al. c-Myc antisense oligonucleotides preserve smooth muscle differentiation and reduce negative remodelling following rat carotid arteriotomy. J Vasc Res, 2005,42(3) :214-225.
  • 7Kipshidze N, Iversen P, Keane E, et al. Complete vascular healing and sustained suppression of neointimal thickening after local delivery of advanced c-myc antisense at six months followup in a rabbit balloon injury model. Cardiovasc Radiat Med,2002,3(1) :26-30.
  • 8Kelman Z. PCNA: structure, functions and interactions Oncogene, 1997,14(6) : 629-640.
  • 9Miniati DN, Hoyt EG, Feeley BT, et al. Ex vivo antisense oligonucleotides to proliferating cell nuclear antigen and Cdc2 kinase inhibit graft coronary artery disease. Circulation, 2000,102(19 Suppl 3): Ⅲ237-Ⅲ242.
  • 10Chandiwal A, Balasubramanian V J, Baldwin ZK. Gene therapy for the extension of vein graft pateney: A review. Vase Endovascular Surg, 2005,39(1) : 1-14.

二级参考文献3

  • 1陈良万,中华心血管病杂志,1995年,23卷,294页
  • 2史玉林,中华医学杂志,1994年,74卷,632页
  • 3张聪,中华外科杂志,1990年,28卷,470页

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