中药复方参麦对大鼠急性肺损伤氧自由基的干预

Interventional effect of compound Shenmai on oxygen free radical in rats with acute lung injury

目的:观察急性肺损伤时氧自由基水平的变化、病理改变、肺水肿程度,分析中药复方参麦在大鼠急性肺损伤中的抗氧自由基作用。方法:实验于2005-10/2006-01在吉林医药学院完成。纯种Wistar大鼠72只,数字表法随机分为治疗组和对照组,每组36只;每组再均分为6组,分别在6,8,12,16,24,36h共6个时间点采集实验数据。各组采用一次性大鼠尾静脉注射油酸0.27mL/kg制作急性肺损伤模型。治疗组:给予参麦注射液(河北省神威药业有限公司,国药准字号Z13020888,10mL/支,为中药复方,主要成分为人参和麦冬)2mL/只,腹腔内注射,每12h给药1次;对照组:用等量生理盐水取代参麦注射液同样方法给药。用检测丙二醛、超氧化物歧化酶、谷光甘肽、谷光甘肽过氧化物酶来评定氧自由基水平;用肺系数评定急性炎症和肺水肿程度;用右肺尖组织200倍光镜病理学检查观察炎症浸润程度。观察不同时间点各指标的变化。结果:所有实验大鼠72只均进入结果分析。①治疗组、对照组组间各时间点肺系数无明显差异,治疗组与对照组相比亦无明显差异(P>0.05);治疗组、对照组组间各时间点丙二醛含量差异明显(P<0.01),治疗组高峰值在24h,对照组在12h和36h;治疗组、对照组组间各时间点T-超氧化物歧化酶含量差异明显(P<0.01),治疗组和对照组变化趋势基本一致;治疗组谷光甘肽组间各时间点差异无显著性意义(P>0.05),但除16h外其他时间点较对照组水平明显升高(P<0.05);治疗组、对照组谷光甘肽过氧化物酶组间各时间点差异明显,参麦注射液干预后水平呈上升趋势(P<0.05)。②200倍光镜下观察,治疗组6h部分肺泡间质内嗜中性粒细胞聚集和浸润;12h部分肺泡壁充血增厚,较多淋巴细胞及嗜中性粒细胞浸润,肺泡减少;24h其病理变化与12h组相近;36h管腔内淤血,肺泡壁充血增厚,较多嗜中性粒细胞浸润,片状实变。对照组6h肺泡间质淤血、水肿,嗜中性粒细胞聚集和浸润;12h部分肺泡壁增厚,肺泡腔减少,部分实变;24h广泛性嗜中性粒细胞浸润,出现较大实变区;36h大片实变区。结论:参麦注射液可抗氧自由基,能有效地降低丙二酮水平,提高超氧化物歧化酶、谷光甘肽和谷光甘肽过氧化物酶水平,缓解急性肺损伤。

AIM： To observe the change of oxyradical level, pathological change and degree of lung edema in rats with acute lung injury （ALl） and analyze the effect of Chinese Shenmai against oxygen free radicals in ALl rats. METHODS： The experiment was conducted at Jilin Medical College from October 2005 to January 2006. Totally 72 purebred Wistar rats were randomly divided into treatment group and control group with 36 rats in each group. Each group was assigned into 6 subgroups. The experimental data were collected at hours 6, 8, 12, 16, 24 and 36, respectively. 0.27mL/kg 9-octadecenoic acid was injected into vena caudalis of rats once to establish the ALl models. The rats in the treatment group were given Shenmai parenteral solution （Jilin Provincial Shineway Pharmaceutical Co., Ltd., No. Z13020888, 10 mL in each solution, compound Chinese herb, main component is ginseng and ophiopogonis）, 2 mL in each one, by intrapefitoneal injection, once administration in every 12 hours. The rats in the control group were treated with saline instead of Shenmai parenteral solution. The level of oxygen free radical was assessed by examining malondialdehyde （MDA）, superexide dismutase （SOD）,λ-glutamylcysteinylglycine （GSH） and glutathione perexidase （GSH-Px）. The acute inflammation and degree of pulmonary edema was assessed by calculating lung coefficient. The level of inflammatory infiltration was observed with pathological examination of tissues from the apex of fight lung under light microscope. Change of every index at different time point was observed. RESULTS： A total of 72 experimental rats were involved in the result analysis. ①The lung coefficient had no significant difference in any groups （P 〉 0.05 ）. The level of MDA had significant difference between treatment groups and control groups at any time point （P 〈 0.01）. In the treatment groups the peak value appeared at hour 24, while in the control groups it was at hours 12 and 36. The difference was significant in SOD between treatment groups and control groups at any time point （P 〈 0.01）. The change tendency between treatment groups and control groups was coincident mostly. There was insignificant difference in GSH in treatment groups at any time point （P 〉 0.05）, but it was increasing significantly in the control groups except at hour 16 （P 〈 0.05）. Between the treatment groups and control groups there were significant differences in GSH-Px at any time points. The level increased after intervening with Shenmai parenteral solu- tion （P 〈 0.05）. ②Under 200 power light microscope, part of neutrophil in alveoli pulmonum matrix were gather and infiltrated at hour 6 in the treatment groups. Part of alveolar wall engorged with blood and thickened at hour 12, and many leukomonocytes and neutrephil were infiltrated with less pulmonary alveoli. At hour 24 the pathological change was similar to that at hour 12. At hour 36 there was congestion in lumens, many infiltrated neutrephils, and lamellar consol; The alveolar wall was engorged with blood and thickened. In the control groups at hour 6 there were congestion and hydroncus in alveoli pulmonis mesenchyma, aggregation and infiltration of neutrephil. At hour 12 part of alveolar wall thickened, less alveolar space, partial consol. At hour 24 there was widely neutrephil infiltration and largely consol region. At hour 36 there was largely consol region. CONCLUSION： The Shenmai parenteral solution can resist oxygen free radical, decrease effectively the level of MDA, increase the levels of SOD,GSH and GSH-Px, and alleviate ALI.