康复训练对急性脑梗死大鼠行为学及神经生长因子及脑源性神经营养因子的影响

Influence of rehabilitation training on ethology, nerve growth factor and brain-derived neurotrophic factor in rats after acute cerebral infarction

目的:观察康复训练对急性脑梗死大鼠脑功能恢复及脑内神经生长因子、脑源性神经营养因子的影响。方法:实验于2001-11/2002-04在哈尔滨医科大学动物实验中心进行。①将30只大鼠随机分为假手术组、造模组和康复组3组(n=10),造模组和康复组大鼠用线栓法制备脑缺血动物模型,假手术组不栓塞。②康复组大鼠每天置于滚筒式网状训练器内进行转动训练,平衡木上行走训练,转棒上转动训练及网屏抓握训练,共40min;其他两组大鼠不干预。③各组大鼠在术后24h、3,7d分别以Bederson评分评估神经功能(0～3分,评分越高,神经功能缺陷越重)、平衡木(0～5分,评分越高,功能越差)、转棒(0～3分,评分越高,功能越差)、网屏测评(0～3分,评分越高,功能越差)评估其运动功能,并于术后7d处死大鼠,测定各组大鼠脑组织内的神经生长因子及脑源性神经生长因子阳性细胞数目。结果:经补充后30只大鼠进入结果分析。①Bederson评分:造模组和康复组在各个时间点均高于假手术组(P<0.01)。②平衡木、转棒和网屏测评结果:术后3,7d造模组和康复组各项评分均高于假手术组(P<0.01),康复组术后3d平衡木测试、术后7d所有测试得分均低于造模组(P<0.05或0.01)。③无论脑皮质还是海马,康复组神经生长因子及脑源性神经生长因子阳性细胞数目均显著高于假手术组和造模组(P<0.01)。结论:康复训练能提高脑梗死大鼠平衡、行走及抓握能力,增加患肢肌力,并能诱导缺血周边区神经生长因子及脑源性神经营养因子的表达。

AIM： To observe the effects of rehabilitation training on cerebral functional restoration, nerve growth factor of brain and brain-derived neurotrophic factor in rats after acute cerebral infarction. METHODS： The experiment was carried out in the Center for Animal Experiment, Harbin Medical University from November 2001 to April 2002. ①Totally 30 rats were randomlydivided into 3 groups, sham operation group （group A）, model group （group B） and rehabilitation group （group C） （n=10）. Cerebral ischemic animal models were made with thread embolism method in the group B and the group C, while those in the group A were not treated with embolism. ②The rats of the group C were put into trommel reticulate trainer every day to do writhing training, walking training on balancing wood, writhing training on rotating bar and prehension training on net screen for 40 minutes, while the rats in the other groups were not intervened. ③Bederson was used to assess neural function （0-3 points, the higher score represented more severe neural functional defect） at the 24 hours, the 3^rd day and the 7^th day after operation, respectively. The balancing wood （0-5 points, the higher score represented the worse function）, rotating bar （0-3 points, the higher score represented worse function）, and net screen （0-3 points, the higher score represented worse function） were applied to evaluate the motor function. At the 7^th day the rats were killed, and the number of positive cells of nerve growth factor in cerebra tissue and brain-derived neurotrophic factor of rats in each group was measured. RESULTS： After compensation, a total of 30 rats were involved in the result analysis. ①Bederson score： It was higher in the group B and the group C than that in the group A at each time point （P 〈 0.01 ）. ②Results of balancing wood, rotating bar and net screen： Scores of each item in the group B and the group C were higher than that in the group A at the 3^nd and 7^th days after operation （P 〈 0.01 ）. Result of balancing wood at the 3^nd day after operation, results of all the tests at the 7^th day in the group C were all lower than that in the group B （P 〈 0.05 or 0.01 ）. ③No matter the pallium or hippocampus, the number of positive cells of nerve growth factor and brain-derived neurotrophic factor of rats in group C was significantly higher than that in the group A and the group B （P 〈 0.01 ）. CONCLUSION： The rehabilitation therapy can improve the ability of equilibrating, walking, grasping, and strengthen the myedynamia, as well as induce the expression of nerve growth factor and brain-derived neurotrophic factor.