骨髓间质干细胞移植对大鼠颅脑损伤后氧化应激的影响

Effect of MSCs' transplantation on oxidative stress after traumatic brain injury in adult rats

目的观察骨髓间质干细胞移植对大鼠外伤性脑损伤的治疗效应和对大鼠颅脑损伤后氧化应激的影响。方法体外分离培养MSCs;建立大鼠TBI模型;经颈内动脉移植MSCs,观察大鼠神经功能的改善状况和两组大鼠脑组织内氧化应激指标的差异。结果MSCs在体外可长期培养扩增,生物学特性稳定;经颈动脉移植4周后,移植治疗的大鼠其神经功能状况改善较好。与对照组相比较,实验组大鼠脑组织内SOD的活性较高,羟自由基的含量较低。结论移植MSCs对TBI所造成的大鼠神经功能缺损有一定的治疗效应。而移植MSCs后能上调脑组织的抗氧化能力,这可能是产生治疗效应的机制之一。

Objective To investigated the effect of bone marrow stromal cells （MSCs） administered by the way of internal carotid artery on functional outcome after traumatic brain injury in adult rats. Besides,we observed the influence of anti--oxidation after transplantation. Methods MSCs were harvested from rat bone marrow. A controlled cortical impact was delivered to 38 adult rats to induce traumatic brain injury, and 24 hours after injury, MSCs were injected into the internal carotid artery of the rats （n = 21）. Every rat was administered 2×10^6MSCs. 16 rats received PBS in the same way. Neurological function was evaluated according to the modified neurological severity score. In addition,not only the content of SOD.OH^- in the experimental group rats brain tissue have been detected,but also the contrast group rats. Results Treatment with 2 ×10^6MSCs significantly improved the rats＇ functional outcomes（P〈0. 05）. After transplantation, higher activity of SOD be detected in experimental group rats brain tissue, on the contrast, content of OH^- were lower. Conclusion These data suggest that MSCs may be a potential therapy for traumatic brain injuries in rat.