MMP-1及TIMP-1在压力性尿失禁患者耻骨宫颈筋膜中的表达

MMP-1 and TIMP-1 expression in pubocervical fascia from patients with stress urinary incontinence

目的探讨女性压力性尿失禁(stress urinary incontinence,SUI)患者盆底胶原代谢状态,分析胶原代谢与疾病发生的关系。方法选择因SUI而行手术治疗患者24例,同期选取因妇科良性疾病行全子宫切除、无压力性尿失禁或盆腔脏器脱垂(pelvic floor prolapse,POP)患者24例作为对照。于手术中取患者耻骨宫颈筋膜,采用免疫组化的方法观察基质金属蛋白酶-1(m atrix m etalloprote inase-1,MMP-1)、组织金属蛋白酶抑制物-1(tissue inh ib itor of m etalloprote inase-1,TIMP-1)表达。结果SUI患者耻骨宫颈筋膜中MMP-1表达显著高于对照组(P<0.05),TIMP-1表达显著低于对照组(P<0.05)。结论SUI患者盆内筋膜胶原代谢活跃,胶原的分解增加可能是SUI发生机制之一。

Objective To investigate collagen metabolism in stress urinary incontinence （SUI） so as to determine the alteration that contributes to SUI. Methods Biopsy specimens of vaginal wall tissue from 24 women with stress urinary incontinence were collected, with matched specimens from 24 female patients without SUI. Immunohistochenical technique were applied to detect the expression of MMP-1 and TIMP-1. Results SUI group demonstrated a significant increase in MMP-1 expression and a decrease in TIMP-1 expression, with significant difference with control group. Conclusion Patients with SUI were in the predominant status of collagen breakdown, which may contribute to the pathogenesis of SUI.