摘要
钙荧光探剂测量活细胞胞浆游离Ｃａ２＋浓度的方法在钙研究中已成为一种越来越重要的技术。特别是由于新的一代荧光探剂的合成和激光共聚焦显微镜的发展，使其应用更加广泛。由于国内使用这种技术的实验室逐渐增多，本文将系统介绍钙荧光探剂的发展、测量原理和方法、新的常用钙荧光探剂的比较及其在生命科学中的应用。
t is
well known that calcium is the key second intracellular
messenger.Techniques using calcium fluorescent probes have been
rapidly developed during the last decade,and have become one of the
most important methods to measure intracellular free
ca2+concentrations. This trend was even more accelerated after the
development of a new generation of calcium fluorescent probes and the
laser scanning confocal microscopy In this article we will review the
development of calcium fluorescent probes,the mechanisms and methods
of measuring intracellular free ca2+ concentrations,the properties of
new calcium fluorescent probes, and their applications in life
sciences.
出处
《生理科学进展》
CAS
CSCD
北大核心
1996年第1期37-42,共6页
Progress in Physiological Sciences
关键词
细胞
钙荧光探剂
钙离子浓度
细胞学技术
Calcium fluorescent probes
Intracellular Ca2+
concentration
Laser scanning confocal microscopy
