兔肝炎性假瘤模型的研制

Establishment of Hepatic Pseudotumor Model in Rabbits

研究临床应用高功率聚焦超声治疗肝肿瘤的有效性和安全性时,需建立合适的大动物肝肿瘤模型。为此,对15只新西兰兔剖腹肝内注射Freund完全佐剂,1周后肝炎性假瘤发生率达100%。5只兔在B超引导下穿刺注射,肝炎性假瘤发生率为80%。本模型的特点是:①肿瘤发生率高;②周期短;③影像学可检测,B超、CT和MRI均有特征性改变;④复制容易。因此,兔肝炎性假瘤模型可较好地评价高功率聚焦超声的定位准确性。

In order to evaluate the therapeutic efficacy of high intensity focused ultrasound (HIFU) for treatment of human liver cancer,a suitable large animal model bearing liver tumor is indispensible. Hepatic pseudotumors in 15 rabbits were established by operative injection of 1—2ml Freund's complete adjuvant into the desirable site of the liver,the pseudotumors were developed in all the animals 7 days after the injection. Another 5 rabbits were injected percutaneously under sonographic guidance,4 of them developed pseudotumors. Autopsies revealed that the pseudotumor was a well-formed lesion of approximately lcm in diameter in the liver. Histologically, the lesion was an inflammatory granuloma. This model showed the following advantages: ① easy to be induced with a high successful rate; ② a short latent period; ③ the tumor can be detected easily by sonography,CT and MRI, and showed the characteristic changes in these imaging modalities.