老年学习记忆减退大鼠脑突触体膜流动性改变

Change of Brain Synaptosomal Membrane Fluidity in Aged Learning- and Memoryimpaired Rats

选用Ｍｏｒｒｉｓ水迷宫将老年大鼠分为学习记忆正常和学习记忆减退两部分，采用荧光偏振技术，对青年、老年记忆正常和老年记忆减退鼠脑分离实触体膜流动性进行测定，并检测神经节苷脂ＧＭ１对膜流动性的影响．结果表明老年记忆减退鼠新皮质、海马结构突触体膜荧光各向异性明显增加，即膜流动性显著降低，ＧＭ１对膜流动性有明显改善作用．相关分析表明新皮质、海马结构实触膜流动性与老年学习记忆减退密切相关，ＧＭＩ的积极作用为临床治疗提供实验依据．

Aged rats were divided into aged memory-impaired rats and normal aged rats based on their behaviour in the Moms water maze. Brain synaptosomal membrane fluidity and the effect of GM1 on it was evaluated by fluorescence polarization technique. The results showed that statistically significant decreases of the synaptosomal membrane fluidity in the neocortex and hippocampal formation were found in the aged memory-impaired rats compared with the young and normal aged rats, and that GM1 could ameliorate the abnormal membrane fluidity. Correlation analysis indicated that the brain synaptosomal membrane fluidity was closely related to aged learning and memory impairment. It was suggested that GM1 has a potential therapeutic value for aged memory impairment through altering neuronal membrane structure.