抗BmNPV即刻早期蛋白三联ribozyme在家蚕细胞中的表达

Expression of Triplet Ribozyme Targeting Bombyx Mori Nuclear Polyhedrosis Virus (BmNPV) Immediate Early Gene mRNA in Bm-N Cell

为了阻断家蚕核多角体病毒（ＢｍＮＰＶ）的基因表达，以ＢｍＮＰＶ的即刻早期蛋白基因（ＩＥ）为靶序列，设计了三联ｒｉｂｏｚｙｍｅ．体外切割反应表明，该ｒｉｂｏｚｙｍｅ能特异地切割靶序列的ｍＲＮＡ；细胞实验表明，细胞中表达的ｒｉｂｏｚｙｍｅ也能够特异地切割靶序列，从而使受ＢｍＮＰＶ感染的Ｂｍ－Ｎ细胞中的多角体减少约３０％．

In order to evaluate the effect of ribozymes on suppressing BmNPV, triplet ribozyme (R426) targeting the BmNPV immediate early gene (IE) was designed. In vitro cleavage experiment showed that R426 transcript was able to cleave the target sequence specifically. Expression experiment indicated that R426 extracted from Bm-N cell can also cleave the target specifically and cause about 30% reduction of BmNPV polyhedra in transfected cell.