改良硫代巴比妥酸荧光法测定血清过氧化脂质

Improvement of the Thiobarbituric Acid Fluorescence Analysis of Serum Lipoperoxides

对目前使用的过氧化脂质（ＬＰＯ）硫代巴比妥酸（ＴＢＡ）荧光测定法进行了改进．血清样本在冰醋酸存在的条件下，１００℃水解６０ｍｉｎ，ＬＰＯ释放的雨二醛（ＭＤＡ）与ＴＢＡ形成复合物，用甲醇沉淀血清蛋白质并减少非特异性干扰，以λ＝５１５ｎｍ、λｅｍ＝５５０ｎｍ测定ＭＤＡ－ＴＢＡ复合物的荧光强度．此法操作简便、快速，结果准确，荧光稳定．ＬＰＯ含量（以ＭＤＡ表示）在０～２０μｍｏｌ／Ｌ与荧光强度呈线性关系（ｒ＝０．９９９４），最低检测限为０．１６μｍｏｌ／Ｌ，回收率为１０５．０８％，重复性ＣＶ＝４．２３％．

The thinbarbituric acid(TBA)fluorescence spectrophotometry of lipoperoxides(LPO),at present used for research,has been improved. This assay of serum LPO involves a hydrolysis in a diluted glacial acetic acid solution at 100℃ for 60 min and the hydrolysis product malondialdehyde (MDA) forms complex with TBAthe methanol precipitation of serumproteins reduces nonspecific interference.The fluorecence intensity of the MDATBA complex is measured at excitation wavelength 515nmThe method is simple,rapid accurate and steady The fuorescence intersity is positively correlatvie to the LPO concentration in the range of 0～20μmol/L The minimum detection limkt is 0.16μmol/L The average recovery rate is 105.8 % Precision (CV)is 4023%