全自动生化分析仪测定血清乳酸脱氢酶同工酶I

Studies on the Measurement of Lactate Dehydrogenase Isoenzyme I Activities by Using Automatic Analyzer

应用α－糜蛋白酶和盐酸胍作为抑制剂选择性测定待测样品中乳酸脱氢酶同工酶Ｉ（ＬＤ－１）．采用蛋白水解酶和抑制剂来破坏乳酸脱氢酶同工酶２～５（ＬＤ２～５）的活性，因此不需预处理待测样品，可直接用于自动生化分析仪．使用日立７１５０分析仪建立了ＬＤ－１分析仪建立了ＬＤ－１测定参数，方法变异系数（ＣＶ）２．７％～４．４％；测得结果（ｙ）与ＬＤ－１电泳方法（ｘ）相关良好，ｙ＝０．９６７ｘ－１．９５７，ｒ＝０．９９２（ｎ＝３７）；检测了１９７名健康男女血清样品，确定ＬＤ－１参考值范围为２９．７８～５９．２６Ｕ／Ｌ，±ｓ＝（４４．５８±７．３９）Ｕ／Ｌ．

A very simple method to determine the activity of LD isoenzyme I by using αchymotrypsin and guanidine has been developed.LD-3～LD-5 can be completely inactivated and LD-2 can be partially inactivated byαchymotrypsin.Guanidine can further completely inactivate LD-2. This method has been used in automatic analyzer. The coefficient of variation of the proteolytic method is among 2.7% ～4.4%.Results obtained by the present method correlates with those by the electrophoresis method (r=0.992, y=0.967,x=1.957).In the present assay system,the reference value for LD-1 activity in healthy people ranges from 29.78～59.26 U/L.The study showed that the selective measurement of LD-1 isoenzyme method is rapid and accurate.It has great clinical significance.