细菌铁蛋白释放铁的动力学研究

STUDIES ON KINETICS OF IRON RELEASING FROM BACTERIAL FERRITIN

棕色固氮菌细菌蛋白在可见光谱区中有定性的特征吸收峰。细菌铁蛋白经过量Ｎａ２Ｓ２Ｏ４还原后，该蛋白的α、β和Ｓ峰的吸光度随着蛋白还原程度增大而递增。细菌铁蛋白的氧化还原状态可分为氧化态、半还原态和深度还原态。细菌铁蛋白铁核中的磷铁组成存在着非均匀性，该蛋白释放铁核表层的铁的反应为一级反应，推测这一过程受蛋白壳中的血红素调控。细菌铁蛋白释放铁核内层的铁的反应为零级反应。

Bacterial ferritin from Azotobacter vinelandii has qualitative characteristic absorption peaksin the visible spectral region. After redued by excess Na2S2O4, the absorbance of bacterialferritin in the α.β and S peaks increased as the degree of reduction enhance α. The bacterialferritin existed in oxidized, half-reduced, or full-reduced state. The phosphate-ironcomposition in the iron core of the bacterial ferritin heterogeneous and the kinetic reactionof the iron release from bacterial ferritin was the first-order on the surface of itsiron core, this process might be controlled by the hare in the protein shell, as well as that ofthe release of iron from inside the iron core of the bacterial ferritin was the zero-older.