顺磁探针16－NS和荧光探针DPH在DPPG交插脂双层中的行为研究

THE BEHAVIOR OF FATTY ACID SPIN LABLE 16-NS AND FLUOROPHORE DPH IN INTERDIGITATED DPPG BILAYER INDUCED BY ANISODAMINE

研究了１６—ＮＳ和ＤＰＨ在被山莨菪碱诱导形成交插脂结构的ＤＰＰＧ脂质体中的行为。在交插脂双层结构中，标记在脂酰链末端的１６—ＮＳ的序参数明显增大，即同标记在靠脂酰链头部的５—ＮＳ的序参数的差距缩小。这些颇磁探针在交插脂双层结构中的运动比在非交插脂双层结构中受到更大的限制。ＤＰＨ荧光强度在ＤＰＰＧ由非交插脂双层转变为交插脂双层结构时急剧下降。引起这种下降的原因可能是膜脂在从非交插脂双层结构向交插脂双层结构的转变过程中使得ＤＰＨ处于一个更为亲水的环境中。

In the preset study the behavinr of 16-NS and DPH in DPPG inedigitated bilayer induce by anisodalnine was investigatal. Intetdigitation caused a large inCreaSe in the orderparameter of a fatty aam spin fabled near the terminal methyl, 16-NS, so that it approacheStO that of another fatty acki spin fabled closer tO the polar lead group region, 5-NS. Themotion of these spin labels borne more restrital in an interdigitatal gel phase bilayer thanin a noninteldigitatal.The fluousrm inboity of DPH wus ndutal as DPPG underwent the bilayer transitionfrom noninterdigitatal tO interdigitatal. The decrease in fluorescence may rebate to a change inlocation of the probe in the membrane during the transition.