梭曼对大鼠摄食中枢的抑制作用

INHIBITORY EFFECT OF SOMAN ON FEEDING CENTER IN RATS

采用双侧下丘脑外例区（摄食中枢）局部给药的方法，观察梭曼对大鼠摄食中枢的作用。实验结果表明，双侧下丘脑摄食中枢各注入梭曼３μｇ，给药当日大鼠平均摄食量下降６０．９％，抑制作用持续３ｄ，与给药前比较，差别显著（Ｐ＜０．０１或０．０５）；各注入阿托品０．１ｍｇ，平均摄食量下降４１．０％，第２天后恢复正常。胆碱酪酶重活化剂ＨＩ－６与梭曼同时注入下丘脑摄食中枢，或者中枢注入梭曼后立即肌注阿托品、美加明，均可部分对抗梭曼引起的中枢性摄食抑制。说明梭曼抑制大鼠摄食中枢与乙酰胆碱酯酶及乙酰胆碱受体确实有密切关系。

The method of injecting drug directly into bilateral hypothalamic feeding centers was used toobserve the effect of soman on feeding center in rats.The experimental results showed that theamount of food consumed was reduced by 60.9%,43.1%and 40.6% in average on the first 3days respectively after injection of soman 3μg into bilateral hypothalamic feeding centers.Whenatropine 0.1mg was injected by the same route,the amount of food consumed was reduced by41.0% and returned to normal level on the second day.If reactivator HI-6(6μg)and soman(3μg)were injected together into hypothalamic feeding centers,and atropine(1.0mg/kg)ormecamylamine(1.0mg/kg)was given by intramuscular administration immediately after somaninjection,the effect of soman-induced central inhibition of food intake was decreased on the firstday and abolished after the second day.These results indicate that soman-induced central inhibi-tion of food intake in rats is closely related to the inhibition of acetylcholinesterase inhypothalamic feeding center and both muscarinic and nicotinic cholinergic receptors are involved.