肽基脯氨酰异构酶(PIN1)在肝癌组织中的表达

PIN1 expression in human hepatocellular carcinoma

目的为进一步阐明肝细胞癌发生过程中肽基脯氨酰异构酶(Peptidyl-proplylIsomerase,PIN1)作用的分子机制,本实验检测了PIN1在肝细胞癌及相应癌旁组织中的表达差异。方法选取经外科手术切除及病理学证实的肝细胞癌患者的癌组织标本及相应正常癌旁组织,应用逆转录多聚酶链反应(RT-PCR)方法,在mRNA水平上进行检测。并运用统计学方法将其表达水平与患者临床特征进行比较。结果48例肝细胞癌的样本中有33例癌组织中PIN1的表达明显高于癌旁组织,总数的69%(33/48);而PIN1表达量的高低与肿瘤的分期(P>0.05)、转移(P>0.05)、合并肝硬化(P>0.05)、AFP(P>0.05)、HBsAg(P>0.05)、瘤体大小(P>0.05)、有无包膜(P>0.05)均无明显的相关性。结论PIN1在肝细胞癌组织中上调表达,并与肝癌的分期相关。

Objective To understand the molecular mechanism of hepatocellular carcinoma and to investigate the expression of the Peptidyl-proplyl Isomerase （PIN1） in hepatocellular carcinoma and normal tissue, Methods Forty eight hepatocancinoma specimens and matched normal tissues were chosen at the surgical were examined by reverse transcript polymerase chain reaction（RT-PCR） analysis at the level of mRNA, and correlation of its expression level with the clinicopathological features were analyzed by statistical method, Results PIN1 was shown to be overexpressed 67% （33/48） of HCC, and its overexpression have no clear correlation with HCC Edemondson＇s grade （P〉0. 05）, gender （P〉0.05）, translocate （P〉0. 05）, AFP（P〉0.05）, HBsAg （P〉0.05）, size （P〉0.05）, fibrous capsule （P〉0. 05）, emblus （P〉0.05）, Conclusion PIN1 overexpression in hepatocellular carcinoma, and maybe correlated with HCC Edemondson＇s grade.