摘要
目的探讨细胞色素P450 1A1和2D6基冈多态性与慢性苯中毒易感性的关系。方法采用病例-对照研究,选择152名苯中毒工人为病例组,152名接触苯而无中毒表现的工人为对照组。采用限制性片段聚合酶链反应(PCR—RFLP)技术榆测CYP1A1基因3’端非编码区Msp I和CYP2D6第1外显子c.188位点、g.212位点多态性。结果携带CYP1A1 Msp I T/T基因型的个体发生苯中毒的危险性是携带有CYP1A1 Msp I T/C或C/C基因型的个体的1.32倍(95%CI:1.05~1.65, P=0.02);在不吸烟的人群中,携带CYP1A1 Msp I T/T基因型的个体发生苯中毒的风险性是携带T/C或C/C基因型的个体的1.56倍(95%CI:1.15~2.12,P=0.003);携带有CYP2D6 c.188 C/C或C/T基因型个体发生苯中毒的危险性足携带有CYP2D6 c.188 T/T基因型的个体的1.23倍(95%CI:1.05~1.42,P=0.01),在不吸烟的人群中,携带CYP2D6 c.188 C/C或C/T基因型个体发生苯中毒的危险性是携带有CYP2D6 c.188 T/T基因型的个体的1.23倍(95%CI:1.04~1.47,P=0.01)。未发现研究对象的CYP2D6g.212位点存在多态性。结论携带CYP1A1 Msp J T/T基因型、CYP2D6 c.188 C/C和C/T基因型个体对苯中毒可能易感。
Objective To explore the relationship between genetic polymorphisms of CYP-IAI and CYP2D6 and risks of chronic benzene poisoning( BP). Methods A case control study was conducted. 152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were involved. Polymerase chain reaction followed by restriction fragment length polymorphism(PCR-RFLP) technology was used for detecting the single nucleotide polymorphisms(SNPs)of Msp 1 in the non-coding region of CYP-IAI gene and c. 188, g. 212 position in the first extron of CYP2D6 gene. Results The individuals with CYPIA 1 Msp [ T/T genotype had a 1.32 times(95% CI:I .05 - 1.65, P = 0.02) increased risk of BP compared with those carr), ing T/C and C/C genotypes.In no-smoking population, there was a 1.56 times(95% CI: 1.15 - 2.12, P = 0.003)increased risk of BP for subjects carrying CYPIAI Msp [ T/T genotype compared with those carrying T/C and C/C genotypes.The individuals carrying CYP2D6c. 188 C/C or C/T genotype had a 1.23 times(95% CI : 1.05 - 1.42, P = 0.01 ) increased risk compared with those carrying T/T genotypes. In no-smoking population, there was a 1.23 times(95% CI: 1.04 - 1.47, P = 0.01 )increased risk of BP for subjects carrying CYP2D6c. 188 C/C or C/T genotypes compared with those carrying T/T genotype .The single nucleotide polymorphism of g. 212 position in the first extron of CYP2D6 gene had not been validated. Conclusion The individuals with CYP2D6 c. 188 C/C, CYP2D6 c. 188 C/T and CYPIAI Msp [ T/T genotypes tend to be more susceptible to benzene toxicity.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2006年第5期266-269,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金资助项目(30271113)
国家科技部"973"项目(2002CB512902)
关键词
苯中毒
多态性基因
生活方式
Benzene poisoning
Genetic polymorphism
Life style