溴氰菊酯对大鼠脑组织某些抗氧化酶、γ-谷氨酰半胱氨酸合成酶和NF-E2相关因子2基因表达的影响

Effects of deltamethrin on gene expression of some antioridase, gamma glutamylcysteine synthetase and NFE2 related factor 2(Nrf2) in brain tissue

目的研究溴氰菊酯(DM)对大鼠大脑皮层和海马组织铜锌超氧化物歧化酶(CuZn- SOD)、谷胱苷肽还原酶(GR)、γ-谷氨酰半胱氨酸合成酶(γ-GCS)轻亚单位(GCSl)重亚单位(GCSh)基因和红系核因子2(NF-E2)相关因子2(Nrf2)基因表达的影响。方法将18只大鼠随机分为3组,每组6只,对照组腹腔注射橄榄油,低剂量DM染毒组(3．125 mg／kg体重)和高剂量DM染毒组(12．500 mg／kg体重)大鼠染毒5 d。分别用逆转录聚合酶链式反应(RT-PCR)方法测定CuZn-SOD、GR、GCSI、GCSh和Nrf2基因mRNA表达的相对量。12只大鼠随机分为3组,用免疫组织化学方法检测海马和大脑皮层中γ-GCS重亚单位蛋白(GCS-HS)和Nrf2蛋白水平。结果DM染毒大鼠大脑皮层和海马组织中的CuZn-SOD、GR、GCSh和Nrf2基因mRNA水平均未见明显改变;12．500 mg／kg组大脑皮层、3．125 mg／kg组大脑皮层和海马组织中的GCSl基因mRNA水平低于对照组中各相应组织的水平,分别降低至对照组mRNA水平的71．1％、63．6％和75．2％,差异均有统计学意义(P<0．01);DM染毒的大鼠海马CA1、CA2、CA3、齿状回和大脑皮层中的GCS-HS和Nrf2蛋白水平均未见明显改变。结论DM对GR及CuZn-SOD基因,mRNA表达未见影响;DM抑制大鼠海马和脑皮层中GCSl基因mRNA表达,而未见影响GCSh基因mRNA的表达;本实验条件下DM对大脑皮层和海马组织中的Nrf2表达无明显影响。

Objective To study the effects of deltamethrin（DM） on the mRNA expression of copper-zinc dependent SOD （ CuZn- SOD ）, glutathione reductase （GR） and gamma glutamylcysteine synthetase （ γ- GCS ） light subunit（GCS1）, as well as on expression of both mRNA and protein of γ-GCS heavy subunit（GCSh） and NFE2 related factor 2（Nrf2） in cerebral cortex and hippocampus of rats. Methods Eighteen Wistar male rats were randomizedly divided into three groups, six for each group. The low dosage and high dosage DM treated groups were administrated intraperitoneally with DM（the daily dosage was 3. 125,12.500 mg/kg BWT respectively） for five consecutive days while the control group was administered intraperitoneally with olive oil. The relative amount of mRNA expression of these genes was measured by the method of reverse transcription polymerase chain reaction （RT-PCR） （n = 6）. The protein level was detected by the method of immunohistochemistry and image analysis system（ n =4）. Results There was no change in mRNA expression level of CuZn-SOD,GR,GCSh and Nrf2 gene in both cerebral cortex and hippocampus tissue in rats administrated with DM. However,the mRNA level of GCSI gene in cerebral cortex of high dosage group as well as in both cerebral cortex and hippocampus of the low dosage group was significantly lower than that in corresponding tissue in the control group,and was decreased to 71.1% ,63.6% and 75.2% of mRNA level of corresponding tissue in the control group（ P 〈 0.01 ） .There was no obvious effect on protein level of both GCSh and Nrf2 in CA1, CA2, CA3 and dentate gyros（DG） of hippocampus as well as on that in cerebral cortex in rats treated with DM. Conclusion Under the experimental conditions, there is no obvious effect in the mRNA expression level of CuZn-SOD,GR gene,as well as on expression of both mRNA and protein of Nrf2 gene in both cerebral cortex and hippocampus tissue in rats administered with DM. DM depresses the mRNA expression of GCSI gene, but does not affect the mRNA expression of GCSh gene.