铝对大鼠脑组织脂质过氧化作用的影响及其性别差异

Effects of aluminum on Upid peroxidation in rat's brain and its sex - related difference

目的研究铝对大鼠脑组织脂质过氧化作用的影响及其性别差异,为进一步探讨铝的神经毒作用机制提供依据。方法将40只SD大鼠分为4组,每组10只,雌雄各半,每组大鼠分别腹腔注射0．0、2．0、4．0、8．0 AL／kg,连续3 d,间隔1 d,共60 d。用跳台法测试其学习记忆能力的改变,用试剂盒测定其脑组织中的超氧化物岐化酶(SOD)活力和丙二醛(MDA)含量的改变,同时采用透射电镜观察其脑组织超微结构的变化。结果各组不同性别大鼠的各项指标的改变之间差异无统计学意义(P>0．05)。与对照组相比,染铝组大鼠的学习记忆能力明显降低,差异有统计学意义(P<0．05),脑组织内SOD活力降低、MDA含量增高,差异有统计学意义(P<0．05)。电镜观察可见染铝组大鼠大脑皮层神经细胞的膜结构破裂、溶解、消失。结论铝可能通过增强大鼠脑组织的脂质过氧化反应而产生神经毒作用,尚不能认为该作用有性别差异。

Objective To explore the mechanisms of aluminum（Al） - induced neutrotoxicity by studying the effect of aluminum on lipid peroxidation in rat＇ s brain and its sex related difference. Methods Forty SD rats, both male and female, were exposed to aluminum through intraperitioneal injection of AlCl3 solution for 60 days at different dose. After exposure, the step down test was performed to examine the learning and memory abilities of rats, and the activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in rat＇s cerebrum were detected by chemical method. The changes of ultrastructure in cortex were observed by transmission electron microscopy. Results The differences between the changes of all indexes of female and male rats in the same dose-group wasn＇t statistically significant （P 〉 0.05）. Compared with the rats in the control group, the learning and memory abilities of the Al - exposed rats were significantly decreased （P 〈 0.05） .The content of MDA was increased（P 〈 0.01 ） while the activity of SOD was decreased（ P 〈 0.05）. The membrane structure of neurons in cerebrum cortex of the Al - exposed rats were broken, dissolved and gone. Conclusion Aluminum can accelerate lipid peroxidation in rat＇s brain, which may be one of the important intoxication mechanisms of aluminum. However, the sex - related difference of this effect have not yet been observed.