桑树叶片愈伤组织的诱导及不定芽分化影响因素的研究

Studies on Factors Affecting Callus Induction and Adventitious Buds Differentiation From Leaf Culture in Mulberry

通过正交试验,探讨了植物生长调节剂噻二唑苯基脲(Th id iazuron,TDZ)、α-萘乙酸(NAA)、桑品种、叶龄、叶位等5种因素对桑树叶片愈伤组织诱导的影响。筛选出了桑树叶片愈伤组织诱导的最佳条件为:21 d苗龄的陕305组培苗叶片,培养基MS+1.0 mg/L TDZ+0.2 mg/L NAA。在此条件下,陕305愈伤组织诱导率可达93.9%。对得到的陕305叶片愈伤组织进行了不定芽分化诱导,结果表明,最佳分化培养基为MS+3.0 mg/L 6-BA+0.1 mg/L IAA+2%果糖,愈伤组织不定芽分化率高达100%。

Using orthogonal experiment design, the effect of Thidiazuron（TDZ） and α-Naphthalencacetic acid （NAA） content in the medium, effect of the order of leaf, the age of leaf and the varieties of mulberry on the leaf callus induction was investigated. Meanwhile, the effect of 6-benzylaminopurine（BA）, indole-3-acetic acid（IAA） and sugar on callus differentiation was also studied. The results showed that the best condition for callus induction is： age of the mulberry cultured in vitro, 21 d; medium, MS（ Murashige ＆ Skoog medium） ＋ 1.0 mg/L TDZ ＋0.2 mg/L NAA; and that the medium for adventitious bud differentiation of the callus is. MS ＋ 3.0 mg/L 6-BA ＋0. 1 mg/L IAA, fructose, 2%, Under these conditions, the leaf callus induction rate and adventitious bud differentiation rate for Shan 305 reached 93.9% and 100% respectively.