摘要
为研究30K蛋白抑制细胞凋亡的作用,利用RT-PCR从家蚕总RNA中扩增到30KC19基因,对其进行了序列分析并克隆到杆状病毒转座载体pFastBacHTb中,构建成重组转座载体pFastBacHTb-30KC19。利用杆状病毒(Bac-to-Bac)表达系统筛选重组杆状病毒,在昆虫细胞TN5中进行了表达和蛋白检测。结果表明30K蛋白在昆虫细胞中得到了高效表达。
To investigate the effects of the Bombyx moil 30K protein on anti-apoptosis, the 30KC19 gene was cloned by RT-PCR and was sequenced. The analysis results showed that the 30KC19 gene was 771 base pairs long. The fragment of 30KC19 gene has been inserted into a transfer vector pFastBacHTb and the recombination baculovirus was obtained. The 30KC19 protein was expressed in Trichoplusiani (TN5) cells with the recombination baculovirus and the 30KC19 protein was highly expressed.
出处
《蚕业科学》
CAS
CSCD
北大核心
2006年第2期169-173,共5页
ACTA SERICOLOGICA SINICA
基金
国家重点基础研究发展计划"973"项目(编号2005CB121005)
江苏省教育厅高校科学研究项目(编号02KJD18003)
江苏大学自然科学创新预研基金项目(编号04CX08)
关键词
家蚕
30K蛋白
表达
杆状病毒
Bombyx mori
30K protein
Expression
Baculovirus