盐酸吡格列酮对高脂诱导大鼠胰岛素抵抗的干预作用

Study on the prevention of insulin resistance of pioglitazone in the rats induced by high-fat diet

目的:探讨盐酸吡格列酮对大鼠胰岛素抵抗(IR)的预防作用。方法:45只雄性wistar大鼠分为对照组、高脂组和吡格列酮组。对照组喂饲普通块料,高脂组喂饱和脂肪酸提供59%热卡的高脂饲料,盐酸吡格列酮组在喂饲高脂饲料的同时,灌胃给盐酸吡格列酮10mg·kg-1·d-1。各组共喂饲11周后测定糖耐量、空腹胰岛素、胰岛素耐量、胰岛素抵抗指数、血脂等。结果:高脂组大鼠从第4周开始体重明显升高,糖负荷30min、60和120min血糖、糖耐量试验中葡萄糖曲线下面积、皮下注射胰岛素后40min、90min、120min血糖和胰岛素耐量试验中葡萄糖曲线下面积、血清胰岛素、胰岛素抵抗指数、血清胆固醇和甘油三酯含量均较正常对照组明显升高。高脂组大鼠内脏脂肪相对含量比正常对照组显著升高(P<0.001)。盐酸吡格列酮组体重、糖负荷后血糖等方面较高脂组明显改善。结论:盐酸吡格列酮能预防高脂诱导IR的发生,其机制与降低血清胆固醇和甘油三酯含量及促使脂肪组织重新分布有关。

Objective： To investigate the prevention effect of instdin resistance by pioglitazone in the rats induced by hightfat diet. Methods： 45 males Wistar rats including normal group, high fat-fed group, pioglitazone group were used in study. The control rats were fed with common diet. The high fat-fed group were fed with high fat diet containing 59% saturated free fatty acids. The rats of pioglitazone group were fed with pioglitazone 10mg·kg^-1·d^-1 with high fat diet. These items were measured in each group at week 11. Results： （ 1 ） Compared with the normal group, the body weight at week 4 and blood glucose after 30min, 60 and 120min in oral glucose,the tolenrance test area under the glucose curve, blood glucose after 40min, 90 and 120min of insulin injection subcutaneously and the area under glucose curve in the insulin tolerance,serum insulin, insulin resistance index, serum leptin, serum cholesterol, serum triglyceride and the relative content of visceral fat were significantly increased in high fatfed group. （2） Compared with these of fat-fed group, the body weight and oral glucose test were decreased in pioglitazone group. Pioglitazone had no effect on fat denaturation of pancreatic tissue. Conclusion： Pioglitazone could prevent the development of insulin resistance of in the rats induced by high-fat diet, and its mechanism was involved the decreasing of serum cholesterol and triglyceride, redistribution of fat tissue.