无菌条件下野蔷薇种子快速催芽的研究

Study on Seed Germination of Rosa Multiflora in Sterile Condition

野蔷薇种子休眠深,发芽极不整齐,给育苗带来很大麻烦。以野蔷薇的3个无刺品系No.3、No.4、No.11和野蔷薇变种荷花蔷薇为试材,研究在无菌条件下,冷藏时间、GA3浓度和不同基质对野蔷薇种子发芽的影响,探索快速得到无菌实生苗的条件。结果表明:(1)影响野蔷薇种子萌发最主要的因素是冷藏时间;(2)3个无刺品系种子在冷藏10～15d、GA30.05～0.1mg/L处理时发芽率均在90%～100%,而荷花蔷薇种子在冷藏15d、GA30.05mg/L处理时最高发芽率为73%;(3)选用沙子作基质比蛭石更适合种子发芽。

The paper probed into the impact of cold different substrates to speeding up sterile germination storage time, gibberellic acid concentration and by using four varieties of Rosa multiflora seed （R. multiflora f.carnea,R.multiflora＂ inermis No.3＇, R.multiflora＇ inermis No.4＇ and R.multiflora＇ inermis No. 11＇）.According to the tests, we find that：（1）cold storage time is the most important factor for seed germination; （2）the germination of three inermis varieties can be up to 90 to 100 percent on 10 to 15 days cold storage and 0.05 to 0.1 mg/L gibberellic was just at 73 percent; （3）on acid concentration, but the maximal germination of R. substrates, the sand is more suit for germination than the vermiculite.