好食脉孢霉拮抗细菌的分离与鉴定

Isolation and Identification of Bacteria of Antagonism against Neurospora sitophila

从桉树枯叶中分离到4株(ED018、ED013、ED015、ED014)对好食脉孢霉(Neurosporasitophila)具有拮抗作用的细菌。它们在Martin平板上对崎生长36h后,抑菌圈分别平均为26.2mm、24.1mm、23.8mm和19.0mm,与多菌灵有极显著性的差异。细菌菌株表现明显的抑制好食脉孢霉孢子的萌发作用,在棉籽壳自然基质培养基上,也表现明显的拮抗作用,对多种常见食用菌菌丝生长无抑制作用,对曲霉有抑制作用,对常见多种污染霉菌无抑制作用。4株细菌菌株均表现为革兰氏阳性,杆状,具芽孢,运动,结合其生理生化反应特征,鉴定4株菌株均属于芽孢杆菌属(Bacillusspp.)。

There were four strains （ED013, ED014, ED015 and ED018） isolated from dry leave of Euca/ypts that could antagonize to Neurospora sitophila. After growing on the martin＇s plates, their inhibition zones followed by ED018, ED013, ED015, ED014 were 26.2mm, 24.1mm, 23.8mm, 19.0mm respectively, and there was no significant difference among them, but all of them had significant difference with N-（benzimidazolyl-2） methyl carbamate. The ED018 could inhibit the spore germination of N. sitophila and the mycelial growth on the cotton shell media. In another way it didn＇t work to the growth of most of common edible fungus expect Volvariella vovacen, and it did to Aspergillus niger but most of common fungus causing contamination. 4 strains all were identified as Bacillus.