基因芯片检测人肺鳞癌和肺腺癌基因表达的异同

DNA chip analysis of gene expression patterns in human lung squamous cell carcinoma and lung adenocarcinoma

目的用基因芯片技术检测肺鳞癌和肺腺癌基因表达的异同。方法提取人肺鳞癌和肺腺癌组织及正常肺组织的RNA,分别用Cy5-dCTP或Cy3-dCTP标记,再与4096点基因芯片杂交,检测肺鳞癌和肺腺癌组织基因表达的异同。结果肺鳞癌和肺腺癌表达共同上调的基因17条,共同下调的基因19条;肺鳞癌表达显著高于肺腺癌的基因20条,显著低于肺腺癌的基因14条。结论多基因参与肺癌发病,基因芯片技术是肺癌基因表达检测的有效方法。

Objective To study the gene expression patterns in human lung squamous cell carcinoma and adenocarcionma with cDNA chip. Methods The mRNA was extracted from cancer tissue and normal lung tissue, and then was labeled by Cy5-dCTP or Cy3-dCTP. The mixed probes were hybridized to cDNA chip containing 4096 genes and to study the gene expression patterns in lung squamous cell teen genes were up-regulated and 19 genes were down-regulated carcinoma, the expression of 20 genes were remarkable higher carcinoma and adenoearcionma. Results Sevenin both lung squamous cell carcinoma and adenoin squamous cell carcinoma than that in adenocarcinoma and the expression of 14 genes were remarkable lower in squamous cell carcinoma than that in adenoeareinoma. Conclusion Many genes are possibly involved in the initiation and progression of human lung cancer; eDNA chip technique might be a useful method in screening lung cancer implicated genes.