松杨栅锈菌的ITS序列和微卫星分析

ISSR Marker and ITS Sequence Study of Melampsora larici-populina

目的比较和分析中国松杨栅锈菌与国外该菌在ITS序列上的差异和中国该菌种内群体分化状况。方法对来自中国不同地域的松杨栅锈菌的5个生理小种11个菌系核糖体ITS序列和微卫星序列多态性进行了研究。结果中国菌系同英国菌系ITS序列同源性高(99.8%),同法国、加拿大、德国的菌系同源性略低(99.5%),中国菌系间ITS序列完全相同。ISSR标记表明,供试的11个菌系可区分为西部、北方两大菌群。小种C2同C4、C1、C3小种遗传多样性差异显著,其余小种间无显著差异。小种分化与遗传分化不一定相符,C2小种在遗传上表现单一。结论该菌ITS序列高度保守,不适合种下阶元划分,各菌系ITS序列无显著差异。ISSR标记适合该菌种内群体遗传分析,中国松杨栅锈菌存在西部和北方两大菌群。

[ Objective ] An experiment was condnced to compare the differences of ITS-nrDNA lied between the China isolates and foreign isolates of Melampsora larici-populina, [Method] ITS sequences and ISSR markers based on 11 isolates of 5 races collected from different parts in China were studied. [Results] There were no differences in the ITS sequence among 11 China isolates. ITS sequence of China isolates was more homogeneous with that of UK than that of France, German and Canada. ISSR marker study showed that the tested 11 isolates can be divided into western population and northern population. Genetic diversity index of race 2 was significantly different from race 4, race 3 and race 1, No significant difference was found among the other races. Race origination division was not harmonize with its genetic division, except race 2. [ Conclusion] ITS region is conservative, and ITS sequence was not fit for studying differences among races. ISSR marker can be used for intra-species population study, and Melampsora larici-populina in China can be divided into two populations.