甘蓝型油菜Polima和Shan 2A CMS的orf224基因的序列分析

Sequence Comparison on Gene orf224 of Polima CMS and Shan2A CMS in Brassica napus L.

目的油菜PolimaCMS的线粒体基因组中含有orf224的4.5kb的区段是与细胞质雄性不育相关的,验证Shan2ACMS与PolimaCMS在orf224的差异性。方法根据orf224设计5′端和3′端的1对特异引物,对PolimaCMS和Shan2ACMS的线粒体基因组进行PCR扩增,在Shan2A中得到与orf224同源的DNA片段,对其测序分析。结果两序列均由675个碱基组成,核苷酸同源性为99.3%。结论证实Shan2ACMS与PolimaCMS的orf224基因上存在差异,不能简单的从两者具有共同的恢保关系上说明Shan2ACMS就是已知的PolimaCMS。

[ Objective ] The 4.5kb section including gene orf224 in Mitochondrial genomes of Polima CMS in Brassica napus L, is correlated with cytoplasmic male-sterility. [ Method ] To prove the difference of Shan2A CMS and Polima CMS in gene orf224, two special primers of 5′ and 3′ were designed according to gene orf224 in this research. Mitochondrial genomes of Polima CMS and Shan2A CMS were amplified with two primers above. The homologous DNA section of orf224 was gained in Shan2A CMS and sequenced. [Result] The results showed that the two sequences both comprise 675 bases. The homology of the two sequences is 99.3%. [Conclusion] Thus it has proved that Shan2A CMS is different from Polima CMS in gene orf224. So we can＇t take Shan2A CMS as Polima CMS simply from the relation of maintainer and restorer.