紫花含笑(♀)×钙土含笑(♂)杂种F_1代的离体繁殖

In vitro Culture of Hybrid F1 of Michelia crassipes Law(♀)×Michelia calcicola C. Y. Wu ex Law et Y. F. Wu(♂)

当年8月份至次年3月份采集紫花含笑(♀)×钙土含笑(♂)杂种F1代优良单株的顶芽和侧芽,置于H+0.25mg·L-16-BA+0.125mg·L-1NAA+0.10mg·L-1KT+0.05mg·L-1IAA上培养,不定芽诱导率可达115%;诱导的不定芽在H+0.3mg·L-16-BA+0.3mg·L-1KT培养基中增殖及生长较好。经过壮苗后的芽条在H+2.8mg·L-1NAA上的生根率可达100%,且单株根数多,芽条生长健壮。间苯三酚(phloroglucinol)明显促进根的发生,但对根的后期生长有一定的抑制效应。组培苗移栽至红土:腐殖土:珍珠岩=1:1:1的混合基质中,60d时成活率可达90%左右。

The top buds and axillary buds of the hybrid F1 saplings of Michelia crassipes Law （♀）×Michelia calcicola C. Y. Wu ex Law et Y. F. Wu （♂） were collected from August to next March and cultured on the medium H＋0.25 mg.L^-16-BA＋0.125 mg.L^-1 NAA＋0.10 mg.L^-1KT＋0.05 mg.L^-1 IAA, and the rate of adventitious buds reached about 115%. The adventitious buds were cultured on the medium H＋0.3 mg.L^-1 6-BA＋0.3 mg.L^-1 KT, the proliferation and growth were better. When the shoots were cultured on medium H＋2.8 mg.L^-1 NAA, the rooting percentage was 100%. Phloroglucinol could greatly promote the shoot rooting, but it might restrain the root elongation. The well-rooted shoots were transplanted into composts （red soil, humus and perlite mixed with same volume） and the survival rate reached 90% after 60 days.