麻疯树茎段离体培养及快速繁殖研究

Study on tissue culture in vitro and rapid propagation of Barbados nut(Jatropha curcas L.) stem

以麻疯树幼嫩枝条腋芽为外植体．以MS为基本培养基，附加不同浓度的激素组合（6～BA与IBA）进行芽诱导和增殖培养。结果表明，MS基本培养基利于不定芽的诱导与形成，出芽率可达75．0％。附加6-BA2．5mg／L、IBA0．1～0．5mg／L的组合芽增殖率可达47．6％～47．8％；随着6-BA浓度增加，芽增殖率也随着升高，但不同浓度IBA对芽增殖率的影响则没有一定规律，其增殖效果不如6～BA明显。此外，在MS基本培养基中是否添加激素对生根培养影响不大，两者生根率差异不明显。

The axiary bud on young shoot of Barbados nut was used as explants, adding different concentration of plant hormone combinations （6-BA and IBA） on minimal medium （MS）, to carry out buds inducing and multiplication culture. The results showed that MS medium was good for inducing and germinating indefinite buds, and germination rate was 75.0%. The multiplication rate of bud was 47.6% -47.8%, on medium combinations 6-BA 2.5mg/L and IBA 0.1- 0. 5mg/L. The multiplication rate of bud increased with the increase of 6 BA eoneentration, but the effect of different concentration of IBA on multiplication of bud did not show regularity and was not so significant as 6-BA, Otherwise, it had not obvious effect on rootage culture whether adding plant hormone on MS medium or not, there was no significant difference of rootage rate between them.