铝胁迫下外源Ca^(2+)对黑麦幼根膜脂过氧化及保护酶活性的影响

Effect of exogenous Ca^(2+) on membrane lipid peroxidation and the activities of corresponded enzymes in seedling roots of Secale cereale L.under aluminum(Al) stress

以黑麦品种K ing为材料,在铝胁迫条件下研究外源C a2+对黑麦幼根膜脂过氧化及保护酶活性的影响。结果表明,50μm o l/L A l处理后黑麦根系电解质渗漏率和根尖丙二醛(M DA)含量增加,根尖超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性升高。在铝处理液中添加0.5~2.0 mm o l/L C aC l2处理24h后,根系电解质渗漏率、根尖M DA含量减少,根尖SOD、POD、CAT活性显著降低,幼根伸长率显著增加,说明外源钙能降低铝对黑麦的毒害,提高黑麦对铝胁迫的抵御能力,增强细胞膜脂抗过氧化能力。

The effect of exogenous Ca^2＋ on membrane lipid peroxidation and the activities of corresponded enzymes in seedling roots were investigated by using rye （ Secale cereale L. ） （cv. King） as material. The results showed that treatment with 50μmol/L A1 could increase the electrolyte leakage, malondialdehyde （MDA） content and the activities of superoxide dismutase （SOD）, peroxidate （POD） and catalase （CAT） of rye root tips. After adding 0. 5-2.0 mmol/L Ca^2＋ to 50 gmol/L Al^3＋for 24 hours, the electrolyte leakage of roots, MDA content and the activities of SOD, POD and CAT of root tips were decreased obviously, while the root elongation of seedlings was increased obviously. It indicated that the exogenous Ca^2＋ could reduce the toxicity of Al to rye, improve the resistance ability to AI stress, and strengthen the anti-peroxidation ability of membrane lipid.