尝试利用R-Q对应因子分析识别分群融合子代

An Attempt on Using the R-Q Double-factor Analysis Method to Identify and Group Fusants

在特定融合组合的子代群体中,以不同姊妹菌株作为观察样本(n=N),以蛋白质电泳全部谱带泳动率作为指标(p=P),则所对应的谱带光密度扫描峰面积作为其观测值(X)(当某菌株缺乏某条带时,其峰面积记为零),利用R-Q对应因子分析程序,解析这个数据矩阵(X_(nxp))。能够在同一标度的因子平面上考察这一特定融合重组事件发生后,姊妹菌株间和各菌株肉蛋白质谱带位置与含量间以及它们相互之间的多重内在联系,从而推断子代群体与亲本的遗传继承关系。并由此来识别和分群姊妹菌株。这种探索具有拓展数值分类能力和指导融合育种实践的意义。

Among the fusants from the definite fusion-cross, the diffrent sister-stains were regarded as the samples of observation (n = N), and the positions of total bands of protein profiles as the objects (p = P). The photometer-scanning area of the specific band as the experimental value (X) (Zero was taken when the specific band of certain strain was absent). The genetical multi-relationships among the inter-and intra sister-strains in terms of the position and content of protein bands after the fusion recombination occurred from this definite fusion-cross could thus be determined on the same orientational factor-plate by using the computer program of R-Q double-factor method to analysis this data matrix (X)nxp. Therefore the sister-strains could be identified and grouped from the deduced heredityship between the fusants and parents.