加热对IgG结合DNA能力的影响

Heat-aggregated IgG Bind to DNA

加热对ＩｇＧ结合ＤＮＡ能力的影响梁俊峰，郑昌学（清华大学生物科学与技术系，北京１０００８４）聚集免疫球蛋白Ｇ（ＡｇｇｒｅｇａｔｅｄｌｇＧ）是指在某些条件（如温度、自由基等）影响下，ＩｇＧ自身聚集所形成的ＩｇＧ的多聚体．聚集不仅影响ＩｇＧ的免疫反应活性...

Aggregated immunoglobulin G were prepared by heat treatment of human IgG in vitro and the binding of these heat-aggregated IgG to DNA were studied by ELISA. It was found that heat-aggregated IgG,in comparison with normal IgG,are able to interact with DNA. Long-term incubation and high temperature in the range of 25─50℃ favored the yield of IgG with binding capacity to DNA. The experimental results obtained from CD spectra demonstrated that the conformation of heat-aggregated IgG is different from that of normal IgG,and that the interaction of heat-aggregated IgG with DVA can cause the change of DNA secondary structure.