PAK株与PA103株绿脓杆菌外毒素结构基因间的差异及其对功能的影响

Differences between Structural Gene of Pseudomonas Exotoxin from PAK Strain and That from PA103 Strain and Their Effects on its Function

对ＰＡＫ株的绿脓杆菌外毒素结构基因进行全长核苷酸顺序测定时发现：来源于ＰＡ１０３株和ＰＡＫ株的ＰＥ基因一级结构间存在差异，其中Ｔｈｒ^（１７９）（ＡＣＣ）→Ａｌａ^（１７９）（ＧＣＣ）、Ｓｅｒ^（５１５）（ＡＧＣ）→Ｇｌｙ^（５１５）（ＧＧＣ），并有１１个同义突变，但变异并不影响白细胞介素２－绿脓杆菌外毒素融合蛋白的ＡＤＰ－核糖基化活性及其细胞毒活性。

The nucleotide sequence of structural gene of Pseudomonas exotoxin from PAK strain was determined, and its differences from that of PAK103 were first discovered. Besides several consense alteration, there were two amino acid differences such as Thr 179 (ACC) → Ala 179 (GCC) and Sersis (AGC)→ Gly 515 (GGC), but these differences had no effects on ADP-ribosylation and the cytotoxicity of interleukin 2-Pseudomonas exotoxin fusion proteins.