摘要
本研究用大鼠游离的胃平滑肌细胞,观察胃动素对胃平滑肌细胞的收缩作用。结果表明:(1)胃动素明显增强单个胃平滑肌细胞收缩活动,在生理剂量10(-11)─10(-10)mol范围内,呈剂量依赖性。(2)不同胃分区平滑肌细胞对冒动素兴奋反应不同,胃动素对胃窦平滑肌细胞收缩强度大于胃体和幽门。(3)给予抗胃动素血清可以完全取消胃动素对胃肌细胞的收缩反应,而阿托品、TTX、甲氰米胍、loxiglumide均不影响胃动素的作用。(4)给予胞内钙释放阻断剂TMB-8可抑制胃动素对目肌细胞的收缩作用。上述结果提示,胃动素对胃平滑肌细胞的直接作用是由胃动素受体所介导,且与胞内Ca(2+)释放起重要作用。
The present study was conducted to investigate the contractile effect of motilin on isolated smooth muscle cells of rat stomach. The results were as follows: (1)Motilin elicited contraction of gastric smooth muscle cells in a dose-dependent manner from 10(-11) to 10(-10) mol. (2)At concentration of 10-10 mol, the contractile response of antral smooth muscle cells exceeded that of the body and pyrous muscle cells. (3)Anti-motilin serum could completely eliminate the stimulatory effect of motilin, which was not affected by atropine, TTX, cimetidine or loxiglumide. (4)TMB-8, an inhibitor of intracellular Ca2+ release, could also suppress completely the motilin effect.The above results suggested that the motilin effect on isolated gastric smooth muscle cells is a direct one, being mediated by highly specific motilin receptors in connection with the event of intracellular release of Ca(2+).
出处
《生理学报》
CAS
CSCD
北大核心
1996年第2期165-172,共8页
Acta Physiologica Sinica
基金
国家自然科学基金
卫生部科研基金
关键词
胃动素
收缩
胃平滑肌细胞
motilin
contraction
isolated gastric smooth muscle cells
TMB-8