黄姜组培快繁技术试验研究

Studies on Tissue Culture and Rapid Propagation of Dioscorea Zingi Berensis C.H.Wright

分别以黄姜茎段、叶片、块根为外植体，进行无菌系建立、芽分化、生根等试验，探讨黄姜组培快繁技术．结果表明：（1）茎段在①MS＋BA1．0mg．L-1＋NAA0．2mg．L-1（单位下同）；③MS＋BA2．0＋NAA0．2；⑤B5＋BA1．0＋NAA0．2；⑦B5＋BA2．0＋NAA0．2四个培养基上培养，均能诱导出愈伤组织，但尤以⑦号培养基长势最佳；而在这四个培养基上接种的叶片因为全被污染，无愈伤组织形成．（2）块根在②MS＋BA1．0＋NAA0．5；④MS＋BA2．0＋NAA0．5；⑥B5＋BA1．0＋NAA0．5；⑧B5＋BA2．0＋NAA0．5四个培养基上均不能诱导成愈伤组织．（3）愈伤组织在①～⑧号共八个培养基上均能分化出芽，但分化率不一，以③号最高（达100％），④号最低（25．0％）．（4）当丛生芽长至高约3～4cm时，转接到以MS或1／2MS为基本培养基、附加有不同浓度NAA和IBA的培养基中，进行生根培养．生长素NAA与IBA配合均能诱导生根，NAA的浓度增高对根的诱导有抑制作用，而且使苗的生长受阻，出现叶片卷曲现象，用1／2MS与用MS相比，用1／2MS生根更粗壮．（5）将经过炼苗的生根试管苗移栽到混有河沙和砻糠灰（体积比为1：2）的复合基质中，移栽后35d成活率达到87％以上．

Taking stem sections, tender leaves, roots of Dioscorea Zingi berensis C.H. Wright for explants respectively, experiments such as callus formation, buds differentiation, rooting were conducted. The results showed as follows： The first, stem sections were taken explants, callus could be produced in the following 4 mediums including ①MS ＋ BA1.0 mg. L - 1 ＋ NAA0.2 mg. L - 1 （ the unit was the same below） ,③MS ＋ BA2.0 ＋ NAA0.2,⑤B5 ＋ BA1.0 ＋ NAA0.2,⑦B5 ＋ BA2.0 ＋ NAA0.2 among which ⑦B5 ＋ BA2.0 ＋ NAA0. 2 was the best; The second, roots were taken explants, callus couldn, t be produced in the following 4 mediums including ②MS ＋ BA1.0 ＋ NAA 0.5 ;④MS ＋ BA2.0 ＋ NAA0.5 ;⑥B5 ＋ BA1.0 ＋ NAA0.5 ;⑧B5 ＋ BA2.0 ＋ NAA0.5. The third, sprouts could be differentiated from callus in the mediums from No. ① to No. ⑧ among which No.③ ,s differentiation rate was the highest（ 100 % ） and No. ④ , s differentiation rate was the lowest （25.0 % ）. The forth, when sprouts length amounted to 3 - 4 cm, rooting was conducted in MS or 1/2MS medium supplemented with different concentrations NAA and IBA, NAA matching with IBA could induce roots production and high NAA concentration inhibited roots production; roots were more robust in 1/2MS as compared to in MS. The fifth, 35 d after being transplanted, tube -seedlings grew well and the transplantation living rate amounted to up to 87.0 % on the compound substrate composed of fiver sand and long kang＇ash （1：2 by volume） .