ELISA技术筛选200种中草药抗HBsAg的实验研究

AN EXPERIMENTAL INVESTIGATION INTO ASSESSMENT OF 200 CHINESE MEDIC INAL HERBS AGAINST HBSAG USING ELISA TECHNIQUE

使用ＥＬＩＳＡ技术对２００种中草药水提取物进行抗ＨＢｓＡｇ的实验研究，共筛出有效药物７种（占总数的３．５％）。若按５种不同剂量（０．３、０．６、１．２、２．５、５．０ｍｇ／１００μｌ）的药物、２种不同浓度的ＨＢｓＡｇ（１０．９２、１４．２６Ｐ／Ｎ值）与３种不同接触时间（立即、１ｈ、２ｈ）的１０项Ｐ／Ｎ值均数来综合评价药效指数时，７种有效药物的次序为青蒿（１．６７Ｐ／Ｎ值）、大蒜（２．１９Ｐ／Ｎ值）、红孩儿（２．３１Ｐ／Ｎ值）、仙鹤草（２．３１）、魔芋（２．３２Ｐ／Ｎ值）、冬瓜皮（２．６３Ｐ／Ｎ值）和猕猴桃（２．８９Ｐ／Ｎ值）。

With the aid of ELISA technique this schema represented a laboratory approach to the recongnition of anti-HBsAg capability of 200 Chinese medicinal herbs by usiug herbal of extracts.Altogether 7 herbs(3.5%)were identified as effective ones.When forming a multiplex plan by employing 10 ayerage P/N ratios: 5 different doses of herbs (0.3, 0.6,1.2, 2. 5, 5. 5mg/100μl), 2 different concentrations of HBsAg (10.92,14.26 P/N ratio) and 3 different time periods of exposure(immediate,1h,2h), these 7 herbs were listed in the following order: Artemisia apiacea(表蒿)(1.67),Allium sativum(大蒜)(2.19),Begonia laciniata苗(红孩儿)(2.31), Agrimonia pilosa (仙鹤草)(2.31 ), Amorphophallusrivieri (魔芋)(2.32), Benincasa hispida(冬瓜皮)(2.63 ), Actinidiachinensis(猕猴桃)(2.89).