摘要
目的检测尘螨变应原的稳定性。方法利用ELISA技术检测在37℃处理后尘螨变应原的稳定性。结果尘螨变应原溶液在37℃处理6d后,利用人抗血清可以检测到其活性下降,而使用兔抗血清时尘螨抗原生物活性的变化在37℃处理45d后仍未检测到。结论尘螨变应原的稳定性应利用IgE抗体来检测,表明尘螨变应原的生物活性在37℃处理45d的过程中逐渐下降。
Objective To detect the stability of the mite allergen. Methods To detect the biological activity of mite allergen using ELISA assay. Results After the mite allergen products had been placed under 37 ℃ for 6 days, the human anti-sera could detect the decline of their biological activity, but the rabbit anti-sera could not. Conclusions ELISA assay with IgE could evaluate the stabilisty of mite allergens, the result showed that the biological activity of mite allergen, decreased gradually while the mite allergen products were placed under 37℃ for 45 days
出处
《广西医学》
CAS
2006年第5期634-635,共2页
Guangxi Medical Journal
基金
广东省科技计划项目(2005A10901003)
广州市科技计划项目(2000z02501)
