用非放射性原位杂交在同一张切片上同时在光学和电子显微镜下显示的豌豆rDNA的分布(简报)

LOCALIZATION OF PISUM SATIVUM rDNA SIMULTANEOUSLY AT LEVELS OF LIGHT AND ELECTRON MICROSCOPY BY NON-RADIOACTIVE IN SITU HYBRIDIZATION

原位杂交目前已成为研究各类组织或细胞内DNA或RNA定位最有效的手段,特别是在发育生物学领城,对于研究基周的表达和修饰更具有重要意义。

A digoxigenin (DIG)-labelled rRNA probe was used to hybridize in situ to the vibration sections of paraformaldehyde fixed root tips, followed by immunogold staning and silver enhancement treatment After the vibration sections which had undergone in situ hybridization, immunogold staining and silver enhancement were observed and photographed under the light microscope, they were further prepared into ultrathin sections and studied underthe electron-microscope. This method more directly combined the in situ hibridization at level of the light microscope with that at level of the electron microscope, by using a piece of vibration section, through which a more detailed rDNA localization was revealed in interphase nuclei of Pisum sativum. Some advantage and disadvantage about the method is discussed in this paper.