牛FABGL基因cDNA的克隆与序列分析

Study on the cloning and sequence analysis of bovine FABGL gene

以中国西门塔尔牛肝脏组织为材料，运用同源序列克隆技术结合RT-PCR和RACE技术，对牛FABGL基因的cDNA进行了克隆与序列分析。并对推导的FABGL蛋白结构与性质进行了初步分析。结果表明，牛FABGL基因的cDNA序列长994 bp，包括780 bp的开放阅读框、16 bp的5′非翻译区和198bp的完整3′非翻译区，由260个氨基酸组成。该基因cDNA核苷酸编码区序列与猕猴、人、猪和小鼠FABGL基因的相似性分别为89％，89％，87％和86％。推导的氨基酸序列与猕猴、人、猪和小鼠的相似性分别为89％，87％，89％和86％。以FABGL基因cDNA编码区序列构建的分子进化树研究结果表明，牛的FABGL基因在人、猕猴、猪、鼠等物种中，与猪的亲缘关系最近。牛的FABGL蛋白的三级结构包含2个跨膜结构-Cutoff模体，分别位于11～16位氨基酸和128～131位氨基酸处。

The liver tissue from Chinese Simmental cattle was collected to extract RNA and the cDNA of the bovine FABGL gene was determined and analyzed by homology cloning approach combined with RT- PCR, 3′ and 5′ RACE in this study. Sequence analysis and bioinformatics study showed that this cDNA contained 994 bp nucleotides,with a 780 bp open reading frame （ORF） flanked by a 16 bp 5′ UTR （uncompletely） and a 198 bp 3′ UTR. The CDS of the obtained bovine FABGL gene showed 89%,89%,87% and 86% identity with the corresponding macaque,human,pig and rat respectively. The deduced amino acid sequence （260 AA） showed 89 %, 87 %, 89 % and 86 % identity with the corresponding macaque, human, pig and rat respectively. Analyzed results for phylogenetic tree of the amino acid sequences of cattle FABGL and other FABGL proteins in human and other animals showed that,among all species mentioned above, the nearest relationship existed between cattle and pig compared to the relationship between cattle and other species.