反相高效液相色谱法测定豆制品中两种异黄酮甙元的含量

Determination of Two Isoflavone Aglycones in Soy Products by RP-HPLC

建立了反相高效液相色谱测定异黄酮甙元的方法。采用Hypersil BDS C18色谱柱（250×4．6mm i．d．，5μm），流动相为甲醇（A）、0．5％乙酸水溶液（B），流速为1．0mL／min，柱温为35℃，检测波长为260nm，进样量为10μL。结果表明：大豆黄素的线性范围为0．0450～0．3150μg，加标回收率为99．05%，相对标准偏差（RSD）为1．31%；染料木黄酮的线性范围为0．0448～0．3136μg，加标回收率为98．82%，RSD为0．50％。方法可用于豆豉及其它大豆制品中异黄酮甙元的测定。

A new method based on RP-HPLC for determination isoflavone aglycones has been developed. established. With Hypersil BDS C18 （250×4. 6 mm i. d. , 5μm）, the mobile phase was methanol and 0.5% acetic acid solution, the flow rate was 1.0 mL/min, column temperature was 35℃ ,UV-detection was set at 260 nm and the sample size was 10 μL. The results show the linear range of Daidzein is 0. 0450～0. 3150μg,the recovery is 99. 05%, RSD is 1.31%. The linear range of Genistein is 0. 0448～0. 3136μg, the recovery is 98. 82 %, RSD is 0.50 %. The method has been applied to the determination of isoflavone aglycones in lobster sauce and soy products with satisfactory result.