二甲酚橙-锆(Ⅳ)配合物光度法测定蛋白质

Spectrophotometric Determination of Protein by Using Xylenol Orangl-Zr(Ⅳ) Complex

在酸性溶液中，蛋白质与二甲酚橙-锆（Ⅳ）配合物能够相互作用形成超分子复合物，其最大吸收波长较二甲酚橙锆（Ⅳ）配合物红移了50nm。在实验选定最佳条件下，600nm波长处的吸光度与牛血清白蛋白（BSA）、人血清白蛋白（HAS）均在0．2～16μg／mL范围内呈线性关系，相对标准偏差为0．83％（n=7），检出限为0．1mg／L。该法灵敏度高，选择性好，用于人血清总蛋白含量的测定，结果满意。

Xylenol Orangl-Zr（Ⅳ） can react with proteins to produce a super molecular complex Xylenol Orangl Zr（Ⅳ ） BSA in the buffer solution of citrate sodium-HCl at pH = 2.5. Its maximum absorption was at 600 nm with 50 nm of red shift compared to Xylenal Orongl Zr（Ⅳ ） complex. The linear range is from 0. 2 to 16 mg/L for BSA,and from 0. 2 to 16 mg/L for HAS, respectively. The method has been applied to the determination of protein in human serum albumin samples with satisfactory results.